Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Ankyrin-B with ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID : 22131424). The section was incubated with ab307414 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Ankyrin-B with ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cardiac muscle (PMID : 12571597). The section was incubated with ab307414 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Ankyrin-B with ab307414 at 1/50 (10.12 ug/ml) dilution followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor™ 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on rat liver (PMID : 17940615). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307414 for 60 mins at room temperature. The section was then mounted using Fluoromount™. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor™ 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Ankyrin-B with ab307414 at 1/50 (10.12 ug/ml) dilution followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor™ 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307414 for 60 mins at room temperature. The section was then mounted using Fluoromount™. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor™ 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Ankyrin-B with ab307414 at 1/50 (10.12 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor™ 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307414 for 60 mins at room temperature. The section was then mounted using Fluoromount™. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor™ 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Ankyrin-B with ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab307414 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use RABbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Ankyrin-B with ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cardiac muscle. The section was incubated with ab307414 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Ankyrin-B with ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on mouse liver (PMID : 17940615). The section was incubated with ab307414 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Ankyrin-B with ab307414 at 1/50 (10.12 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse liver (PMID : 17940615). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307414 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• WB

Supplier Data

Western blot - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using 307414, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 26109584).

Negative control : liver (PMID : 17940615)

Lysates were freshly made and used immediately to minimize protein degradation.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Ankyrin-B antibody [EPR26550-85] (<a href='/en-us/products/primary-antibodies/ankyrin-b-antibody-epr26550-85-ab307414'>ab307414</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate 20 μg

Lane 2:

Mouse liver tissue lysate 20 μg

Lane 3:

Rat brain tissue lysate 20 μg

Lane 4:

Rat liver tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 160 kDa,220 kDa,260 kDa

true

Exposure time: 81s

• Dot

Supplier Data

Dot Blot - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415)

This data was developed using ab307414, the same antibody clone in a different buffer formulation. Dot blot analysis of Ankyrin-B using ab307414 at 1 : 1000 (0.506 ug/ml) followed by a Goat Anti-RABbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST