Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)

Rabbit Recombinant Monoclonal Ankyrin-B antibody. Carrier free. Suitable for Dot, IHC-Fr, IHC-P, WB and reacts with Transfected cell lysate - Mouse, Rat, Mouse samples.

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Images

Western blot - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (AB307415), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Dot	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	WB
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Expected	Not recommended	Not recommended	Not recommended	Tested	Tested	Tested
Rat	Expected	Not recommended	Not recommended	Not recommended	Tested	Tested	Tested
Transfected cell lysate - Mouse	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Transfected cell lysate - Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Transfected cell lysate - Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Transfected cell lysate - Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Transfected cell lysate - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Transfected cell lysate - Mouse	Dilution info -	Notes -

Target data

See full target information Ank2

Function

Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions (PubMed:19109891). In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites (PubMed:12571597). Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:15262991). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (PubMed:19007774). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).

Alternative names

AnkB, Ank2, Ankyrin-2, ANK-2, Ankyrin-B, Brain ankyrin

Recommended products

Rabbit Recombinant Monoclonal Ankyrin-B antibody. Carrier free. Suitable for Dot, IHC-Fr, IHC-P, WB and reacts with Transfected cell lysate - Mouse, Rat, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR26550-85
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ankyrin-B also known as ANK1 and sometimes referred to as ankyrin 105 is a protein expressed in many tissues with significant presence in the brain heart and skeletal muscle. It has a molecular mass of approximately 215 kDa. This protein interacts with cytoskeletal elements and membrane proteins playing a major role in maintaining the stability and proper function of protein complexes at the plasma membrane.

Biological function summary

Ankyrin-B is essential for the localization and stability of ion channels and transporters. It forms part of a multi-protein complex that regulates the anchoring of these proteins to the cytoskeleton. In neurons ankyrin-B binds to cell adhesion molecules and ion channels affecting their function and distribution which is vital for maintaining cellular communication and structural integrity.

Pathways

Ankyrin-B has significant roles in cardiac and neurological pathways. In the cardiac pathway it interfaces with proteins such as the sodium-calcium exchanger and Na+/K+ ATPase essential for heart contractility and rhythm. In neuronal pathways ankyrin-B interacts with NCAM1 (Neural Cell Adhesion Molecule 1) which affects neuronal growth and guidance.

Associated diseases and disorders

Ankyrin-B is associated with cardiac arrhythmias and neurological disorders. Mutations in ankyrin-B can lead to long QT syndrome a disorder linked with irregular heartbeats. In neurological contexts alteration of ankyrin-B function associates with autism spectrum disorders. It has been found to interact with cardiac proteins like the sodium channel SCN5A which plays a role in maintaining a proper heartbeat.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using 307414, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26109584).
Negative control: liver (PMID: 17940615)
Lysates were freshly made and used immediately to minimize protein degradation.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-Ankyrin-B antibody [EPR26550-85] (Anti-Ankyrin-B antibody [EPR26550-85] ab307414) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate 20 μg
Lane 2: Mouse liver tissue lysate 20 μg
Lane 3: Rat brain tissue lysate 20 μg
Lane 4: Rat liver tissue lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 160 kDa, 220 kDa, 260 kDa
Exposure time: 81s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum. The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cardiac muscle. The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse liver (PMID:17940615). The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum (PMID: 22131424). The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/4000 (0.127 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cardiac muscle (PMID: 12571597). The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat liver (fresh) tissue lABeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/50 (10.12 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on rat liver (PMID:17940615). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat cerebrum (fresh) tissue lABeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/50 (10.12 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse liver (fresh) tissue lABeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/50 (10.12 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse liver (PMID:17940615). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse cerebrum (fresh) tissue lABeling Ankyrin-B with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1/50 (10.12 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Ankyrin-B antibody [EPR26550-85] ab307414 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.
Dot Blot - Anti-Ankyrin-B antibody [EPR26550-85] - BSA and Azide free (ab307415)
This data was developed using Anti-Ankyrin-B antibody [EPR26550-85] ab307414, the same antibody clone in a different buffer formulation.
Dot blot analysis of Ankyrin-B using Anti-Ankyrin-B antibody [EPR26550-85] ab307414 at 1:1000 (0.506 ug/ml) followed by a Goat Anti-RABbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

Exposure time: 180 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST