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Mouse Monoclonal Ankyrin-B antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANK2 aa 200-500.

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Images

Flow Cytometry - Anti-Ankyrin-B antibody [N105/13] (AB131419), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin-B antibody [N105/13] (AB131419), expandable thumbnail
  • Western blot - Anti-Ankyrin-B antibody [N105/13] (AB131419), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human ANK2 aa 200-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q01484

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Expected
Tested
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
0.1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1 µg/mL
Notes

1 μg/mL of ab131419 was sufficient for detection of Ankyrin brain in 20μg of Rat brain lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:12571597). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (By similarity). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (By similarity).

Alternative names

ANKB, ANK2, Ankyrin-2, ANK-2, Ankyrin-B, Brain ankyrin, Non-erythroid ankyrin

Recommended products

Mouse Monoclonal Ankyrin-B antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANK2 aa 200-500.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human ANK2 aa 200-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q01484
Clone number
N105/13
Purification technique
Affinity purification Protein G
Specificity

No cross-reactivity against Ankyrin-G

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

The clone number has been updated from S105-13 to N105/13, both clone numbers name the same antibody clone.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ankyrin-B also known as ANK1 and sometimes referred to as ankyrin 105 is a protein expressed in many tissues with significant presence in the brain heart and skeletal muscle. It has a molecular mass of approximately 215 kDa. This protein interacts with cytoskeletal elements and membrane proteins playing a major role in maintaining the stability and proper function of protein complexes at the plasma membrane.

Biological function summary

Ankyrin-B is essential for the localization and stability of ion channels and transporters. It forms part of a multi-protein complex that regulates the anchoring of these proteins to the cytoskeleton. In neurons ankyrin-B binds to cell adhesion molecules and ion channels affecting their function and distribution which is vital for maintaining cellular communication and structural integrity.

Pathways

Ankyrin-B has significant roles in cardiac and neurological pathways. In the cardiac pathway it interfaces with proteins such as the sodium-calcium exchanger and Na+/K+ ATPase essential for heart contractility and rhythm. In neuronal pathways ankyrin-B interacts with NCAM1 (Neural Cell Adhesion Molecule 1) which affects neuronal growth and guidance.

Associated diseases and disorders

Ankyrin-B is associated with cardiac arrhythmias and neurological disorders. Mutations in ankyrin-B can lead to long QT syndrome a disorder linked with irregular heartbeats. In neurological contexts alteration of ankyrin-B function associates with autism spectrum disorders. It has been found to interact with cardiac proteins like the sodium channel SCN5A which plays a role in maintaining a proper heartbeat.

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3 product images

  • Flow Cytometry - Anti-Ankyrin-B antibody [N105/13] (ab131419), expandable thumbnail

    Flow Cytometry - Anti-Ankyrin-B antibody [N105/13] (ab131419)

    Overlay histogram showing SH-SY5Y cells stained with ab131419 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131419, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin-B antibody [N105/13] (ab131419), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin-B antibody [N105/13] (ab131419)

    4% formaldehyde-fixed SK-N-BE cells stained for Ankyrin brain (green) using ab131419 at 1/100 dilution in ICC/IF.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 min room temperature, 5 min room temperature.

  • Western blot - Anti-Ankyrin-B antibody [N105/13] (ab131419), expandable thumbnail

    Western blot - Anti-Ankyrin-B antibody [N105/13] (ab131419)

    Western blot detection of Ankyrin brain in Rat brain membrane tissues using ab131419 at 1:1000 dilution.

    All lanes: Western blot - Anti-Ankyrin-B antibody [N105/13] (ab131419)

    Predicted band size: 430 kDa

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Product protocols

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