AB212053

Anti-Ankyrin erythroid/ANK antibody [S388A-10]

Name: Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053) | Abcam
Brand: Abcam Limited
SKU: AB212053
Price: 520 USD
Availability: InStock

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(4 Publications )

Mouse Monoclonal Ankyrin erythroid/ANK antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANK1.

View Alternative Names

ANK, ANK1, Ankyrin-1, ANK-1, Ankyrin-R, Erythrocyte ankyrin

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (AB212053)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (AB212053)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling Ankyrin erythroid/ANK with ab212053 at 1/100 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin R Antibody (D) Composite.

Supplier Data

Western blot - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (AB212053)

Primary incubation 16 hours at 4°C.

All lanes:

Western blot - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053) at 1/1000 dilution

All lanes:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/200 dilution

Predicted band size: 206 kDa

false

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (AB212053)

4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Ankyrin erythroid/ANK (green, C) using ab212053 at 1/100 dilution in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S388A-10

Isotype

IgG2b

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ANK1.

P16157

Specificity

Does not cross-react with Ankyrin-B or Ankyrin-G.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ankyrin erythroid also known as ANK Ankyrin-R or Ankyrin-1 is a protein with a molecular mass of approximately 215 kDa. This protein is part of the ankyrin family which functions to help maintain cell structure and organization. Ankyrin erythroid is primarily expressed in erythrocytes where it plays an important role in connecting integral membrane proteins to the underlying spectrin-actin cytoskeleton. Its binding capability and mechanical support are essential for the stability and elasticity of the red blood cell membrane.

Biological function summary

Ankyrin erythroid acts as a linker protein and contributes to the structural integrity of the erythrocyte membrane. It is an important component of the ankyrin-spectrin complex which provides necessary mechanical support to the cell's lipid bilayer. The stability offered by this complex is key for erythrocytes' remarkable ability to withstand deformation as they transit through narrow capillaries. Ankyrin erythroid interacts with multiple proteins including band 3 and spectrin supporting cellular health and longevity by maintaining membrane cohesion and surface area.

Pathways

Ankyrin erythroid plays a significant role in the cytoskeletal network and the erythrocyte membrane stabilization pathways. Its interaction with band 3 and spectrin is central in the maintenance of cell surface protein organization and ion transport pathways. These interactions and the structural framework have direct implications on red blood cell function and homeostasis. Ankyrin erythroid's pathway relations emphasize its part in ensuring the flexibility and stability of erythrocyte membranes through its role in maintaining the organization and stability of the cytoskeleton.

Defects or deficiencies in ankyrin erythroid can lead to hereditary spherocytosis an anemia characterized by the presence of spherical erythrocytes that are prone to hemolysis. This condition sees disruptions in the ankyrin-spectrin complex affecting cell elasticity and lifespan. Additionally ankyrin erythroid interacts with proteins like spectrin and band 3 where mutations can exacerbate membrane stability issues further contributing to the pathology of hereditary spherocytosis. Understanding these interactions provides insight into potential therapeutic interventions for related hematological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed : 35835865). Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions.. Isoform Mu17. Together with obscurin in skeletal muscle may provide a molecular link between the sarcoplasmic reticulum and myofibrils.

See full target information ANK1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular cell biology 16: PubMed39367479

2024

Super-resolution microscopy unveils the nanoscale organization and self-limiting clustering of CD47 in human erythrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyu Yang,Fulin Xing,Fen Hu,Mengdi Hou,Hao Dong,Jiayu Cheng,Wan Li,Rui Yan,Jingjun Xu,Ke Xu,Leiting Pan

Journal of clinical laboratory analysis 37:e24763 PubMed36458368

2022

HSPA6, a novel prognostic and therapeutic biomarker, associated with Ming classification in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Zhang,Hui-Qin Zhuo,Zhi-Jun Hong,Jing-Jing Hou,Jia Cheng,Jianchun Cai

EMBO reports 23:e54755 PubMed35642585

2022

Malaria parasites release vesicle subpopulations with signatures of different destinations.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Abou Karam,Irit Rosenhek-Goldian,Tamar Ziv,Hila Ben Ami Pilo,Ido Azuri,Anna Rivkin,Edo Kiper,Ron Rotkopf,Sidney R Cohen,Ana Claudia Torrecilhas,Ori Avinoam,Alicia Rojas,Mattia I Morandi,Neta Regev-Rudzki

Nature communications 12:1172 PubMed33608523

2021

20S proteasomes secreted by the malaria parasite promote its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elya Dekel,Dana Yaffe,Irit Rosenhek-Goldian,Gili Ben-Nissan,Yifat Ofir-Birin,Mattia I Morandi,Tamar Ziv,Xavier Sisquella,Matthew A Pimentel,Thomas Nebl,Eugene Kapp,Yael Ohana Daniel,Paula Abou Karam,Daniel Alfandari,Ron Rotkopf,Shimrit Malihi,Tal Block Temin,Debakshi Mullick,Or-Yam Revach,Ariel Rudik,Nir S Gov,Ido Azuri,Ziv Porat,Giulia Bergamaschi,Raya Sorkin,Gijs J L Wuite,Ori Avinoam,Teresa G Carvalho,Sidney R Cohen,Michal Sharon,Neta Regev-Rudzki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ankyrin erythroid/ANK antibody [S388A-10]

Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (AB212053)

Western blot - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (AB212053)

Immunocytochemistry/ Immunofluorescence - Anti-Ankyrin erythroid/ANK antibody [S388A-10] (AB212053)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise