Anti-Annexin-11/ANXA11 antibody [EPR26004-63]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (AB300577)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Annexin 11/ANXA11 with ab300577 at 1/50 (9.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (AB300577)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HT-1080 (human fibrosarcoma epithelial cell) cells labelling Annexin 11/ANXA11 with ab300577 at 1/50 (9.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HT-1080 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (AB300577)

Annexin 11/ANXA11 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate 10 ug with ab300577 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300577 at 1/1000 dilution.

VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (ab300577) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 10 µg

Lane 2:

ab300577 at 1/30 IP in HeLa whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300577 in HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 56 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (AB300577)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29306955). The bands beneath the target band are likely to be degraded target fragments.

All lanes:

Western blot - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (ab300577) at 1/1000 dilution

Lane 1:

Human ovary cancer tissue lysate

Lane 2:

Human small intestine tissue lysate

Lane 3:

Mouse uterus tissue lysate

Lane 4:

Rat uterus tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 56 kDa

false

Exposure time: 7.75s

• WB

Supplier Data

Western blot - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (AB300577)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 32003509). The bands beneath the target band in lane 1-3 are likely to be degraded target fragments.The lysates of lane 5-6 were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (ab300577) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Lane 4:

A431 (human epidermoid carcinoma epithelial cell), whole cell fresh lysate

Lane 5:

HT-1080 (human fibrosarcoma epithelial cell), whole cell fresh lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 56 kDa

false

• WB

Supplier Data

Western blot - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (AB300577)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 32003509).

All lanes:

Western blot - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (ab300577) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate

Lane 2:

HeLa transfected with siRNA specifically targeti ANXA11, whole cell lysate 20

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 56 kDa

false

Exposure time: 26s