JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151517

Anti-Annexin-13/ANXA13 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal Annexin-13/ANXA13 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ANXA13 aa 1-50.

View Alternative Names

ANX13, ANXA13, Annexin A13, Annexin XIII, Annexin-13, Intestine-specific annexin, ISA

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-13/ANXA13 antibody (AB151517)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-13/ANXA13 antibody (AB151517)

Immunofluorescent analysis of methanol-fixed A431 cells labeling Annexin-13/ANXA13 with ab151517 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-13/ANXA13 antibody (AB151517)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-13/ANXA13 antibody (AB151517)

Immunohistochemical analysis of paraffin-embedded Human Colon carcinoma tissue labeling Annexin-13/ANXA13 with ab151517 at 1/500 dilution.

Western blot - Anti-Annexin-13/ANXA13 antibody (AB151517)
  • WB

Unknown

Western blot - Anti-Annexin-13/ANXA13 antibody (AB151517)

10% SDS PAGE

All lanes:

Western blot - Anti-Annexin-13/ANXA13 antibody (ab151517) at 1/2000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 35 kDa,39 kDa,40 kDa,42 kDa,47 kDa,61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ANXA13 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P27216

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-13 also known as ANXA13 acts mainly as a calcium-dependent phospholipid-binding protein. ANXA13 often mediates interactions between the cell membrane and cytoskeleton. Its molecular weight is around 36 kDa. Expression levels of ANXA13 are higher in intestinal tissues compared to other tissues like the liver or kidney. Researchers also find it commonly in the epithelial cells due to its role in membrane dynamics.
Biological function summary

ANXA13 functions in cellular growth processes apoptosis and signal transduction. It likely interacts with cellular structures like membranes by binding to phospholipids in the presence of calcium ions. ANXA13 does not usually operate alone but may form complexes with other proteins. Though less studied than other annexins ANXA13 contributes to cellular homeostasis essential for efficient functioning of the intestinal cells where its expression is more noticeable.

Pathways

ANXA13 plays a role in calcium signaling and membrane trafficking. It interacts with intracellular phospholipids and aids in the regulation of membrane-associated activity which is significant in endocytosis pathways. ANXA13 potentially shares functional relationships with other annexins like ANXA1 and ANXA5 within these pathways aligning them in coordinating cellular responses to calcium ion changes.

ANXA13 seems associated with specific gastrointestinal diseases and possibly certain cancers. Altered expression of ANXA13 occurs in intestinal inflammation and could impact diseases like Inflammatory Bowel Disease (IBD). Moreover changes in ANXA13 expression or function have implications in colorectal cancer development often in conjunction with other annexins such as ANXA4. Understanding ANXA13's involvement could help in developing therapeutic approaches to manage these conditions efficiently.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Isoform A. Binds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner (PubMed : 27676605, PubMed : 30610115). Half-maximal membrane binding requires about 60 uM calcium. Does not bind to membranes that lack phospholipids with an acidic headgroup (PubMed : 27676605).. Isoform B. Binds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner, but requires higher calcium levels for membrane binding than isoform A. Half-maximal membrane binding requires about 320 uM calcium.
See full target information ANXA13
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB283992

Human Annexin-A13/ANXA13 ELISA Kit

Sandwich ELISA - Human Annexin-A13/ANXA13 ELISA Kit (AB283992)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB151306

Anti-TRIM10 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TRIM10 antibody (AB151306)

  • 0
  • 0
Secondary Antibodies

AB191866

VHH Single Domain Anti-Rabbit IgG Fc (HRP)

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (AB191866)

  • 5
  • 2
Primary Antibodies

AB90806

Anti-Pentraxin 3/PTX3 antibody [MNB1]

Recombinant

Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (AB90806)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com