AB154113

Anti-Annexin-2/ANXA2 antibody

Name: Anti-Annexin-2/ANXA2 antibody (ab154113) | Abcam
Brand: Abcam Limited
SKU: AB154113
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Annexin-2/ANXA2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANXA2 aa 1-250.

View Alternative Names

ANX2, ANX2L4, CAL1H, LPC2D, ANXA2, Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36, PAP-IV

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (AB154113)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (AB154113)

Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling Annexin-2/ANXA2 with ab154113 at 1/200 dilution.
The image in the lower panel was merged with DNA probe.

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Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

10% SDS PAGE

All lanes:

Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution

All lanes:

Rat2 whole cell lysate at 30 µg

Predicted band size: 33 kDa,38 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (AB154113)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (AB154113)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Annexin-2/ANXA2 with ab154113 at 1/500 dilution.

Lab

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

All lanes:

Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

Annexin-2/ANXA2 Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 38 kDa

false

Unknown

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

10% SDS PAGE

All lanes:

Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution

Lane 1:

GL261 whole cell lysate at 30 µg

Lane 2:

NIH 3T3 whole cell lysate at 30 µg

Lane 3:

C2C12 whole cell lysate at 30 µg

Predicted band size: 38 kDa

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Supplier Data

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

All lanes:

Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution

Lane 1:

Non-transfected (-) 293T whole cell lysates at 30 µg

Lane 2:

293T transfected with human Annexin-1/ANXA1 whole cell lysates at 30 µg

Lane 3:

293T transfected with human Annexin-2/ANXA2 whole cell lysates at 30 µg

Predicted band size: 38 kDa

false

Unknown

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

All lanes:

Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

HCT116 whole cell lysate at 30 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ANXA2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P07355

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-2 also known as ANXA2 is a multifunctional protein of approximately 36 kDa. It belongs to the annexin family and is known for its strong calcium-dependent interactions with phospholipid membranes. Commonly expressed in endothelial cells it also appears in various tissues including epithelial cells and certain types of cancer cells. ANXA2 forms a heterotetramer complex with S100A10 also known as p11 which modulates its availability and function. Other names for ANXA2 include its gene designation M298 and its role in complexes often goes by ANXA2R.

Biological function summary

Annexin-2 acts beyond mere cellular structural support. It plays a part in fibrinolysis by acting as a co-receptor for plasminogen and tissue plasminogen activator promoting plasmin generation. The protein is also implicated in membrane trafficking signal transduction and organization of microdomains on cellular surfaces. As part of a complex with S100A10 ANXA2 can bind to cytoskeletal elements facilitating vesicle trafficking and influencing cell shape and motility. This complex assembly hints at ANXA2's importance in maintaining cellular dynamism and interplay with external signals.

Pathways

ANXA2 is integral in the regulation of the plasminogen activation pathway important for fibrinolytic activity. It also participates in the signal transduction pathways influencing cellular responses to stress and growth factors. ANXA2 interacts with various proteins like Src kinase and is involved in processes like the EGFR signaling pathway. This protein often crosstalks with the cytoskeletal network enabling the coupling of membrane dynamics with intracellular signaling cascades.

Annexin-2's dysregulation associates with conditions such as cancer and thrombosis. In cancer overexpression of ANXA2 links with tumor progression invasion and metastasis acting synergistically with proteins like Src to promote oncogenic pathways. In thrombosis ANXA2’s role in fibrinolysis becomes impaired contributing to excessive clot formation. Studies also indicate interactions with plasminogen-related proteins as being central to understanding its pathological roles in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed : 18799458, PubMed : 22848640, PubMed : 24808179).. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.

See full target information ANXA2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:106-122.e9 PubMed34875212

2021

SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity.

Applications

Unspecified application

Species

Unspecified reactive species

Daoud Sheban,Tom Shani,Roey Maor,Alejandro Aguilera-Castrejon,Nofar Mor,Bernardo Oldak,Merav D Shmueli,Avital Eisenberg-Lerner,Jonathan Bayerl,Jakob Hebert,Sergey Viukov,Guoyun Chen,Assaf Kacen,Vladislav Krupalnik,Valeriya Chugaeva,Shadi Tarazi,Alejandra Rodríguez-delaRosa,Mirie Zerbib,Adi Ulman,Solaiman Masarwi,Meital Kupervaser,Yishai Levin,Efrat Shema,Yael David,Noa Novershtern,Jacob H Hanna,Yifat Merbl

The Journal of clinical investigation 129:4657-4670 PubMed31545299

2019

Recombinant annexin A6 promotes membrane repair and protects against muscle injury.

Applications

Unspecified application

Species

Unspecified reactive species

Alexis R Demonbreun,Katherine S Fallon,Claire C Oosterbaan,Elena Bogdanovic,James L Warner,Jordan J Sell,Patrick G Page,Mattia Quattrocelli,David Y Barefield,Elizabeth M McNally

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin-2/ANXA2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (AB154113)

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (AB154113)

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

Western blot - Anti-Annexin-2/ANXA2 antibody (AB154113)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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