AB178677

Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]

RabMAb
Recombinant
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(38 Publications)

Rabbit Recombinant Monoclonal Annexin-2/ANXA2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 38 publications.

View Alternative Names

ANX2, ANX2L4, CAL1H, LPC2D, ANXA2, Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36, PAP-IV

5 Images

Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling Annexin-2/ANXA2 with ab178677 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Immunofluorescent analysis of HeLa cells labeling Annexin-2/ANXA2 with ab178677 at 1/100 dilution (red). DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Exposure time
Lanes 1-3 : 1 minute
Lane 4 : 3 minutes

Lanes 1 - 3:

Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/20000 dilution

Lane 4:

Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SKBR-3 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 38 kDa

false

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
HRP

HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
578 PE

PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
Carrier free

Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13052(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-2 also known as ANXA2 is a multifunctional protein of approximately 36 kDa. It belongs to the annexin family and is known for its strong calcium-dependent interactions with phospholipid membranes. Commonly expressed in endothelial cells it also appears in various tissues including epithelial cells and certain types of cancer cells. ANXA2 forms a heterotetramer complex with S100A10 also known as p11 which modulates its availability and function. Other names for ANXA2 include its gene designation M298 and its role in complexes often goes by ANXA2R.

Biological function summary

Annexin-2 acts beyond mere cellular structural support. It plays a part in fibrinolysis by acting as a co-receptor for plasminogen and tissue plasminogen activator promoting plasmin generation. The protein is also implicated in membrane trafficking signal transduction and organization of microdomains on cellular surfaces. As part of a complex with S100A10 ANXA2 can bind to cytoskeletal elements facilitating vesicle trafficking and influencing cell shape and motility. This complex assembly hints at ANXA2's importance in maintaining cellular dynamism and interplay with external signals.

Pathways

ANXA2 is integral in the regulation of the plasminogen activation pathway important for fibrinolytic activity. It also participates in the signal transduction pathways influencing cellular responses to stress and growth factors. ANXA2 interacts with various proteins like Src kinase and is involved in processes like the EGFR signaling pathway. This protein often crosstalks with the cytoskeletal network enabling the coupling of membrane dynamics with intracellular signaling cascades.

Annexin-2's dysregulation associates with conditions such as cancer and thrombosis. In cancer overexpression of ANXA2 links with tumor progression invasion and metastasis acting synergistically with proteins like Src to promote oncogenic pathways. In thrombosis ANXA2’s role in fibrinolysis becomes impaired contributing to excessive clot formation. Studies also indicate interactions with plasminogen-related proteins as being central to understanding its pathological roles in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed : 18799458, PubMed : 22848640, PubMed : 24808179).. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.

See full target information ANXA2

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8551 PubMed41022690

2025

Brain-cervical lymph node crosstalk contributes to brain injury induced by subarachnoid hemorrhage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jinman Chen,Jie Wang,Wenjing Zheng,Wenhao Ding,Zixin Zhuang,Hao Xu,Wenchao Ding,Tianhao Xu,Linmei Wang,Ning Li,Yongjian Zhao,Qi Shi,Lianping Xing,Yongjun Wang,Qianqian Liang

Journal of translational medicine 23:930 PubMed40826459

2025

Single-cell transcriptomic analysis reveals metastatic and immunosuppressive characteristics in meningioma brain-tumor interface.

Applications

Unspecified application

Species

Unspecified reactive species

Boya Huang,Jinlian Liang,Xiaogen Tang,Yue Zhu,Lijuan Gao,Dongwei Fu,Cheng Wei,Yifang Li,Chao Ke,Hongyi Zhang,Oscar Junhong Luo

Cell communication and signaling : CCS 23:253 PubMed40448170

2025

Rapid and high-yield recovery of plasma-derived extracellular vesicles using modified chromatography with soluble protein depletion for biomarker discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Yang You,Zhengrong Zhang,Samuel Cortes,Son N Nguyen,Prakruthi Vadakattu,Bridgette C Melvin,Sean D Jr Mann,Nibedita Basu Ray,Maria Bregendahl,Pam J McLean,Maria Paz Gonzalez-Perez,Seiko Ikezu,Tsuneya Ikezu

Scientific reports 15:15843 PubMed40328825

2025

ANXA2 regulates mitochondrial function and cellular senescence of PDLCs via AKT/eNOS signaling pathway under high glucose conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Huang,Zejing Qiu,Chunhui Jiang,Qian Fang,Jiaye Wang,Mingfang Han,Yizhao Liu,Zehui Li

Nature cell biology 27:438-448 PubMed39984653

2025

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis K Jeppesen,Zachary C Sanchez,Noah M Kelley,James B Hayes,Jessica Ambroise,Emma N Koory,Evan Krystofiak,Nilay Taneja,Qin Zhang,Matthew M Dungan,Olivia L Perkins,Matthew J Tyska,Ela W Knapik,Kevin M Dean,Amanda C Doran,Robert J Coffey,Dylan T Burnette

CNS neuroscience & therapeutics 30:e70065 PubMed39350328

2024

Comprehensive Proteomic Analysis of Dysferlinopathy Unveiling Molecular Mechanisms and Biomarkers Linked to Pathological Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wang,Xin-Yi Liu,Qi-Fang He,Fu-Ze Zheng,Long Chen,Ying Zheng,Ming-Hui Zeng,Yu-Hua Lin,Xin Lin,Hai-Zhu Chen,Min-Ting Lin,Ning Wang,Zhi-Qiang Wang,Feng Lin

PeerJ 12:e18064 PubMed39308808

2024

ANXA2 promotes osteogenic differentiation and inhibits cellular senescence of periodontal ligament cells (PDLCs) in high glucose conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Huang,Jiaye Wang,Chunhui Jiang,Minghe Zheng,Mingfang Han,Qian Fang,Yizhao Liu,Ru Li,Liangjun Zhong,Zehui Li

Traffic (Copenhagen, Denmark) 25:e12937 PubMed38777335

2024

Apolipoprotein E2 Expression Alters Endosomal Pathways in a Mouse Model With Increased Brain Exosome Levels During Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Y Peng,Braison Liemisa,Jonathan Pasato,Pasquale D'Acunzo,Monika Pawlik,Adriana Heguy,Sai C Penikalapati,Amanda Labuza,Harshitha Pidikiti,Melissa J Alldred,Stephen D Ginsberg,Efrat Levy,Paul M Mathews

Cellular and molecular life sciences : CMLS 81:224 PubMed38769196

2024

Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Gui,Yohan Kim,Santra Brenna,Maximilian Wilmes,Giorgio Zaghen,Chris N Goulbourne,Lennart Kuchenbecker-Pöls,Bente Siebels,Hannah Voß,Antonia Gocke,Hartmut Schlüter,Michaela Schweizer,Hermann C Altmeppen,Tim Magnus,Efrat Levy,Berta Puig

Communications biology 7:99 PubMed38225453

2024

Deep proteomic analysis of obstetric antiphospholipid syndrome by DIA-MS of extracellular vesicle enriched fractions.

Applications

Unspecified application

Species

Unspecified reactive species

Wenmin Tian,Dongxue Shi,Yinmei Zhang,Hongli Wang,Haohao Tang,Zhongyu Han,Catherine C L Wong,Liyan Cui,Jiajia Zheng,Yang Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]

Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

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