Rabbit Polyclonal Annexin-4/ANXA4 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANXA4 aa 1 to C-terminus.
Images
Publications
Filter by
- International journal of molecular medicine 46:2102-21142020p53 and ANXA4/NF‑κB p50 complexes regulate cell proliferation, apoptosis and tumor progression in ovarian clear cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuanjuan Liu et. al.PubMed 33125094
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ANXA4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09525
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Alternative names
Annexin A4, 35-beta calcimedin, Annexin IV, Annexin-4, Carbohydrate-binding protein p33/p41, Chromobindin-4, Endonexin I, Lipocortin IV, P32.5, PP4-X, Placental anticoagulant protein II, Protein II, PAP-II, ANX4, ANXA4
Recommended products
Rabbit Polyclonal Annexin-4/ANXA4 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANXA4 aa 1 to C-terminus.
Alternative names
Annexin A4, 35-beta calcimedin, Annexin IV, Annexin-4, Carbohydrate-binding protein p33/p41, Chromobindin-4, Endonexin I, Lipocortin IV, P32.5, PP4-X, Placental anticoagulant protein II, Protein II, PAP-II, ANX4, ANXA4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ANXA4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09525
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Annexin-4 also known as ANXA4 is a protein with a molecular mass of approximately 36 kDa. It belongs to the annexin family characterized by their ability to bind phospholipids in a calcium-dependent manner. Annexin-4 is widely expressed in various tissues including epithelial cells in the gastrointestinal tract kidney and placenta. This protein associates with the inner surface of the plasma membrane and plays roles in processes like vesicle trafficking and membrane dynamics.
- Biological function summary
The protein functions in cellular processes involving membrane-related activities. It does not appear to be part of a larger stable complex but researchers have observed its interaction with cell membranes and involvement in membrane repair and cell signal transduction. Annexin-4 can mediate interactions between the cytoskeleton and the plasma membrane impacting cell motility and structure.
- Pathways
Annexin-4 functions in the phospholipid signaling pathways particularly influencing pathways related to calcium signaling. In the calcium-dependent signaling pathway Annexin-4 regulates cellular responses by cooperating with proteins such as calmodulin and other annexins contributing to processes like cell proliferation and apoptosis.
- Associated diseases and disorders
Annexin-4 has shown associations with cancer and inflammatory diseases. Abnormal expression of Annexin-4 has been observed in certain cancer types where it may affect tumor metastasis and progression by modifying cellular adhesion and migration. Additionally its interactions with inflammation-related proteins such as interleukins suggest its involvement in inflammatory responses and disorders like inflammatory bowel disease. Researchers continue to explore its potential as a biomarker for early detection and as a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-Annexin-4/ANXA4 antibody (ab153883)
10% SDS PAGE
All lanes: Western blot - Anti-Annexin-4/ANXA4 antibody (ab153883) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 35 kDa, 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody (ab153883)
Immunohistochemical analysis of paraffin-embedded D54MG xenograft labeling Annexin IV/ANXA4 with ab153883 at 1/500 dilution.
Immunoprecipitation - Anti-Annexin-4/ANXA4 antibody (ab153883)
Annexin-4/ANXA4 was immunoprecipitated from HepG2 whole cell lysate using 5 μg ab153883.
The WB was probed with ab153883.
Lane 1: Control IgG IP.
Lane 2: ab153883 IP.
All lanes: Immunoprecipitation - Anti-Annexin-4/ANXA4 antibody (ab153883)
Predicted band size: 35 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody (ab153883)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Annexin IV/ANXA4 with ab153883 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com