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AB153883

Anti-Annexin-4/ANXA4 antibody

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(2 Publications)

Rabbit Polyclonal Annexin-4/ANXA4 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANXA4 aa 1 to C-terminus.

View Alternative Names

ANX4, ANXA4, Annexin A4, 35-beta calcimedin, Annexin IV, Annexin-4, Carbohydrate-binding protein p33/p41, Chromobindin-4, Endonexin I, Lipocortin IV, P32.5, PP4-X, Placental anticoagulant protein II, Protein II, PAP-II

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody (AB153883)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody (AB153883)

Immunohistochemical analysis of paraffin-embedded D54MG xenograft labeling Annexin IV/ANXA4 with ab153883 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody (AB153883)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody (AB153883)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling Annexin IV/ANXA4 with ab153883 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

Immunoprecipitation - Anti-Annexin-4/ANXA4 antibody (AB153883)
  • IP

Unknown

Immunoprecipitation - Anti-Annexin-4/ANXA4 antibody (AB153883)

Annexin-4/ANXA4 was immunoprecipitated from HepG2 whole cell lysate using 5 μg ab153883.

The WB was probed with ab153883.

Lane 1 : Control IgG IP.

Lane 2 : ab153883 IP.

All lanes:

Immunoprecipitation - Anti-Annexin-4/ANXA4 antibody (ab153883)

Predicted band size: 35 kDa

false

Western blot - Anti-Annexin-4/ANXA4 antibody (AB153883)
  • WB

Unknown

Western blot - Anti-Annexin-4/ANXA4 antibody (AB153883)

10% SDS PAGE

All lanes:

Western blot - Anti-Annexin-4/ANXA4 antibody (ab153883) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 35 kDa,37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human ANXA4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09525

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-4 also known as ANXA4 is a protein with a molecular mass of approximately 36 kDa. It belongs to the annexin family characterized by their ability to bind phospholipids in a calcium-dependent manner. Annexin-4 is widely expressed in various tissues including epithelial cells in the gastrointestinal tract kidney and placenta. This protein associates with the inner surface of the plasma membrane and plays roles in processes like vesicle trafficking and membrane dynamics.
Biological function summary

The protein functions in cellular processes involving membrane-related activities. It does not appear to be part of a larger stable complex but researchers have observed its interaction with cell membranes and involvement in membrane repair and cell signal transduction. Annexin-4 can mediate interactions between the cytoskeleton and the plasma membrane impacting cell motility and structure.

Pathways

Annexin-4 functions in the phospholipid signaling pathways particularly influencing pathways related to calcium signaling. In the calcium-dependent signaling pathway Annexin-4 regulates cellular responses by cooperating with proteins such as calmodulin and other annexins contributing to processes like cell proliferation and apoptosis.

Annexin-4 has shown associations with cancer and inflammatory diseases. Abnormal expression of Annexin-4 has been observed in certain cancer types where it may affect tumor metastasis and progression by modifying cellular adhesion and migration. Additionally its interactions with inflammation-related proteins such as interleukins suggest its involvement in inflammatory responses and disorders like inflammatory bowel disease. Researchers continue to explore its potential as a biomarker for early detection and as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
See full target information ANXA4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 46:2102-2114 PubMed33125094

2020

p53 and ANXA4/NF‑κB p50 complexes regulate cell proliferation, apoptosis and tumor progression in ovarian clear cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Liu,Huimin Wang,Mingjun Zheng,Lu Deng,Xue Zhang,Bei Lin

Evidence-based complementary and alternative medic 2019:4050327 PubMed31949467

2019

Hydroxysafflor Yellow A Inhibits TNF--Induced Inflammation of Human Fetal Lung Fibroblasts via NF-B Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Liu,Yan Wang,Huijuan Wen,Xiaofang Sun,Yu Wang
View all publications
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Product promise

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