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AB315196

Anti-Annexin-6/ANXA6 antibody [4G3]

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Mouse Monoclonal Annexin-6/ANXA6 antibody. Suitable for WB, ICC/IF and reacts with Human, Rat, Horse, Dog, African green monkey, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANXA6.

View Alternative Names

ANX6, ANXA6, Annexin A6, 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II, Chromobindin-20, Lipocortin VI, Protein III, p68, p70, CPB-II

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [4G3] (AB315196)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [4G3] (AB315196)

Immunofluorescent analysis of HeLa cells stained with ab315196, dilution 1 : 500 in red, and costained with chicken pAb to vimentin, CPCA-Vim, dilution 1 : 2,000 in green. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while the vimentin antibody produces strong staining of theintermediate filament network of the cytoskeleton.

Western blot - Anti-Annexin-6/ANXA6 antibody [4G3] (AB315196)
  • WB

Supplier Data

Western blot - Anti-Annexin-6/ANXA6 antibody [4G3] (AB315196)

Western blot analysis of different cell lysates using ab315196, dilution 1 : 2,000 in green : [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. ab315196 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to RPCA-HSP60, dilution 1 : 5,000, in red. The band at 60kDa corresponds to HSP60 protein.

All lanes:

Western blot - Anti-Annexin-6/ANXA6 antibody [4G3] (ab315196) at 1/2000 dilution

Lane 2:

NIH-3T3 cell lysate

Lane 3:

C6 cell lysate

Lane 4:

HeLa cell lysate

Lane 5:

HEK293 cell lysate

Lane 6:

A72 cell lysate

Lane 7:

NBL6 cell lysate

Lane 8:

COS1 cell lysate

Observed band size: 60 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4G3

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Horse, Dog, African green monkey, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ANXA6.

P08133

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7 - 8 Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-6 also known as ANXA6 is a member of the annexin family of proteins. It has a mass of approximately 68 kDa and is expressed in various tissues with substantial presence in muscle and liver. This protein acts mechanically by binding to membranes in a calcium-dependent manner playing a role in membrane-associated processes such as endocytosis and exocytosis. Annexin-6 can also interact with cytoskeletal elements influencing cell structure and stability.
Biological function summary

Annexin-6 functions as a versatile scaffold that organizes multiprotein complexes. It modulates membrane trafficking processes and participates in signaling pathways within the cell. This protein can influence intracellular calcium homeostasis by interacting with calcium channels and transporters making it an integral part of cellular signaling and communication.

Pathways

Annexin-6 plays a role in the MAPK and PI3K/Akt signaling pathways. It associates with proteins such as Ras and Protein Kinase C impacting cell proliferation survival and differentiation. These interactions highlight the importance of annexin-6 in cellular responses to external stimuli and help regulate various physiological processes in the body.

Annexin-6 has been linked to cardiac disorders and cancer. In the context of cardiac health it interacts with proteins like phospholamban and calmodulin to influence heart muscle function and calcium regulation. In cancer annexin-6's involvement in the Akt signaling pathway can affect tumor growth and metastasis often interacting with other proteins like EGFR and HER2. This makes it a significant protein of interest in disease research and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
See full target information ANXA6
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Product promise

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