AB226410

Anti-Annexin-6/ANXA6 antibody

Name: Anti-Annexin-6/ANXA6 antibody (ab226410) | Abcam
Brand: Abcam Limited
SKU: AB226410
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Annexin-6/ANXA6 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANXA6 aa 500-600.

View Alternative Names

ANX6, ANXA6, Annexin A6, 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II, Chromobindin-20, Lipocortin VI, Protein III, p68, p70, CPB-II

1 Images

Supplier Data

Western blot - Anti-Annexin-6/ANXA6 antibody (AB226410)

Cell lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Annexin-6/ANXA6 antibody (ab226410) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 76 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human ANXA6 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

P08133

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Giant Panda": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226410 was affinity purified using an epitope specific to Annexin-6/ANXA6 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-6 also known as ANXA6 is a member of the annexin family of proteins. It has a mass of approximately 68 kDa and is expressed in various tissues with substantial presence in muscle and liver. This protein acts mechanically by binding to membranes in a calcium-dependent manner playing a role in membrane-associated processes such as endocytosis and exocytosis. Annexin-6 can also interact with cytoskeletal elements influencing cell structure and stability.

Biological function summary

Annexin-6 functions as a versatile scaffold that organizes multiprotein complexes. It modulates membrane trafficking processes and participates in signaling pathways within the cell. This protein can influence intracellular calcium homeostasis by interacting with calcium channels and transporters making it an integral part of cellular signaling and communication.

Pathways

Annexin-6 plays a role in the MAPK and PI3K/Akt signaling pathways. It associates with proteins such as Ras and Protein Kinase C impacting cell proliferation survival and differentiation. These interactions highlight the importance of annexin-6 in cellular responses to external stimuli and help regulate various physiological processes in the body.

Annexin-6 has been linked to cardiac disorders and cancer. In the context of cardiac health it interacts with proteins like phospholamban and calmodulin to influence heart muscle function and calcium regulation. In cancer annexin-6's involvement in the Akt signaling pathway can affect tumor growth and metastasis often interacting with other proteins like EGFR and HER2. This makes it a significant protein of interest in disease research and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

See full target information ANXA6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:505-519 PubMed37902450

2024

Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Jun Yan,Long Han,Zi-Lin Zou,Ai-Lei Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin-6/ANXA6 antibody

Western blot - Anti-Annexin-6/ANXA6 antibody (AB226410)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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