AB199422

Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal

RabMAb
Recombinant
KO Validated
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(3 Publications)

Rabbit Recombinant Monoclonal Annexin-6/ANXA6 antibody. N-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

ANX6, ANXA6, Annexin A6, 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II, Chromobindin-20, Lipocortin VI, Protein III, p68, p70, CPB-II

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling Annexin-6/ANXA6 with ab199422 at 1/100 followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on K562 cell line is observed (Subcellular Location : Cytoplasm [UniProt]). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).

The negative controls are as follows : -
-ve control 1 - ab199422 at 1/1000 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Annexin-6/ANXA6 with ab199422 at 1/100 followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on HeLa cell line is observed (Subcellular Location : Cytoplasm [UniProt]). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).
The negative controls are as follows : -
-ve control 1 - ab199422 at 1/1000 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

Supplier Data

Immunoprecipitation - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Annexin-6/ANXA6 was immunoprecipitated from 1mg of Raji (Human Burkitt's lymphoma cell line) whole cell extract with ab199422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199422 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Raji whole cell extract 10 μg (Input).

Lane 2 : ab199422 IP in Raji whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199422 in Raji whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)

Predicted band size: 76 kDa

false

Exposure time: 10s

Lab

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Lanes 1 - 4 : Merged signal (red and green). Green - ab199422 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab199422 was shown to react with Annexin-6/ANXA6 in wild-type HeLa cells in western blot with loss of signal observed in ANXA6 knockout cell line ab265677 (ANXA6 knockout cell lysate ab257351). Wild-type and ANXA6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab199422 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ANXA6 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ANXA6 (Annexin-6) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-anxa6-annexin-6-knockout-hela-cell-line-ab265677'>ab265677</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Predicted band size: 76 kDa

Observed band size: 75 kDa

false

Supplier Data

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Blocking and diluting buffer was 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 12140262).

All lanes:

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422) at 1/10000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 76 kDa

Observed band size: 68 kDa

true

Exposure time: 15s

Supplier Data

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Blocking and diluting buffer was 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID : 12140262).

All lanes:

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Mouse spleen tissue lysate at 10 µg

Lane 4:

Rat brain tissue lysate at 10 µg

Lane 5:

Rat heart tissue lysate at 10 µg

Lane 6:

Rat kidney tissue lysate at 10 µg

Lane 7:

Rat spleen tissue lysate at 10 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg

Lane 9:

NIH/3T3 (Mouse embryo fibroblast) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 76 kDa

Observed band size: 68 kDa

true

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17308

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-6 also known as ANXA6 is a member of the annexin family of proteins. It has a mass of approximately 68 kDa and is expressed in various tissues with substantial presence in muscle and liver. This protein acts mechanically by binding to membranes in a calcium-dependent manner playing a role in membrane-associated processes such as endocytosis and exocytosis. Annexin-6 can also interact with cytoskeletal elements influencing cell structure and stability.

Biological function summary

Annexin-6 functions as a versatile scaffold that organizes multiprotein complexes. It modulates membrane trafficking processes and participates in signaling pathways within the cell. This protein can influence intracellular calcium homeostasis by interacting with calcium channels and transporters making it an integral part of cellular signaling and communication.

Pathways

Annexin-6 plays a role in the MAPK and PI3K/Akt signaling pathways. It associates with proteins such as Ras and Protein Kinase C impacting cell proliferation survival and differentiation. These interactions highlight the importance of annexin-6 in cellular responses to external stimuli and help regulate various physiological processes in the body.

Annexin-6 has been linked to cardiac disorders and cancer. In the context of cardiac health it interacts with proteins like phospholamban and calmodulin to influence heart muscle function and calcium regulation. In cancer annexin-6's involvement in the Akt signaling pathway can affect tumor growth and metastasis often interacting with other proteins like EGFR and HER2. This makes it a significant protein of interest in disease research and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

See full target information ANXA6

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 149:51 PubMed40411591

2025

Annexin A6 membrane repair protein protects against amyloid-induced dystrophic neurites and tau phosphorylation in Alzheimer's disease model mice.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine R Sadleir,Karen P Gomez,Abigail E Edwards,Armana J Patel,Makenna L Ley,Ammaarah W Khatri,Joanna Guo,Shreya Mahesh,Elyse A Watkins,Jelena Popovic,Devi Krishna Priya Karunakaran,Dmitry Prokopenko,Rudolph E Tanzi,Bernabe Bustos,Steven J Lubbe,Alexis R Demonbruen,Elizabeth M McNally,Robert Vassar

Matrix biology : journal of the International Society for Matrix Biology 129:44-58 PubMed38582404

2024

The extracellular matrix differentially directs myoblast motility and differentiation in distinct forms of muscular dystrophy: Dystrophic matrices alter myoblast motility.

Applications

Unspecified application

Species

Unspecified reactive species

Ashlee M Long,Jason M Kwon,GaHyun Lee,Nina L Reiser,Lauren A Vaught,Joseph G O'Brien,Patrick G T Page,Michele Hadhazy,Joseph C Reynolds,Rachelle H Crosbie,Alexis R Demonbreun,Elizabeth M McNally

Frontiers in pharmacology 10:1338 PubMed31780948

2019

Cryptotanshinone Attenuates Airway Remodeling by Inhibiting Crosstalk Between Tumor Necrosis Factor-Like Weak Inducer of Apoptosis and Transforming Growth Factor Beta 1 Signaling Pathways in Asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Chongyang Wang,Mingyu Zheng,Yunho Choi,Jingzhi Jiang,Li Li,Junfeng Li,Chang Xu,Zhemin Xian,Yan Li,Hongmei Piao,Liangchang Li,Guanghai Yan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Immunoprecipitation - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (AB199422)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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