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Rabbit Recombinant Monoclonal Annexin-7/ANXA7 antibody. N-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990), expandable thumbnail
  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990), expandable thumbnail
  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990), expandable thumbnail
  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990), expandable thumbnail
  • Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Species
Rat
Dilution info
1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

Alternative names

ANX7, SNX, OK/SW-cl.95, ANXA7, Annexin A7, Annexin VII, Annexin-7, Synexin

Recommended products

Rabbit Recombinant Monoclonal Annexin-7/ANXA7 antibody. N-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16173
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Annexin-7 (ANXA7) also known as synexin is a calcium-dependent phospholipid-binding protein. It has a molecular weight of approximately 51 kDa. This protein is expressed in various tissues with notable expression in the heart and brain. Mechanically ANXA7 plays a role in membrane fusion and exocytosis which are critical processes for cellular communication and transport.

Biological function summary

Annexin-7 functions as an important player in cell proliferation and signal transduction. It forms part of the annexin family of proteins which are known for their role in calcium ion binding and membrane-related activities. Annexin-7 has a role in linking Ca2+ signaling with phospholipid membrane dynamics which suggests its involvement in cellular events like growth and differentiation.

Pathways

Annexin-7 influences both the MAPK and EGFR signaling pathways. These pathways are essential for regulating cell division apoptosis and cellular response to external stimuli. Within these pathways Annexin-7 interacts with proteins such as Ras and PI3K which are important for propagating signals that control cellular behaviors.

Associated diseases and disorders

Abnormalities in Annexin-7 expression and function associate it with cancer and schizophrenia. In cancer altered ANXA7 function may disrupt normal cell communication and growth pathways contributing to tumorigenesis. In schizophrenia disrupted calcium signaling involving ANXA7 may affect neuronal communication and brain function. In these conditions Annexin-7 interacts with proteins like p53 and NMDA receptors which are critical to the pathology of cancer and neurological disorders respectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990), expandable thumbnail

    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    All lanes: Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution

    All lanes: U87-MG cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990), expandable thumbnail

    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    All lanes: Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution

    All lanes: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990), expandable thumbnail

    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    All lanes: Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

    Lane 1: Human fetal kidney tissue lysate at 10 µg

    Lane 2: Human spleen tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990), expandable thumbnail

    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    All lanes: Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

    Lane 1: Mouse kidney tissue lysate at 10 µg

    Lane 2: Mouse spleen tissue lysate at 10 µg

    Lane 3: Rat kidney tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990), expandable thumbnail

    Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    ANXA7 was immunoprecipitated from U87-MG (human glioblastoma) whole cell extract with ab198990 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198990 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: U87-MG (human glioblastoma) whole cell extract (Input) 10 μg.
    Lane 2: ab198990 IP in U87-MG (human glioblastoma) whole cell extract.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab198990 in U87-MG (human glioblastoma) whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue sections labeling ANXA7 with ab198990 at a 1/200 dilution. Goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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