AB166621

Anti-Annexin-9/ANXA9 antibody [EPR11220]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal Annexin-9/ANXA9 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

ANX31, ANXA9, Annexin A9, Annexin XXXI, Annexin-31, Annexin-9, Pemphaxin

2 Images

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Immunoprecipitation - Anti-Annexin-9/ANXA9 antibody [EPR11220] (AB166621)

Immunoprecipitation of Annexin-9/ANXA9 from pellet from Human fetal kidney lysate using ab166621 at 1/10 dilution, followed by WB detection of Annexin-9/ANXA9.

All lanes:

Immunoprecipitation - Anti-Annexin-9/ANXA9 antibody [EPR11220] (ab166621) at 1/1000 dilution

All lanes:

Immunoprecipitation pellet from fetal kidney tissue lysate at 10 µg

Predicted band size: 38 kDa

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Western blot - Anti-Annexin-9/ANXA9 antibody [EPR11220] (AB166621)

All lanes:

Western blot - Anti-Annexin-9/ANXA9 antibody [EPR11220] (ab166621) at 1/1000 dilution

Lane 1:

Human fetal kidney lysates at 10 µg

Lane 2:

Human tonsil lysates at 10 µg

Lane 3:

A431 lysates at 10 µg

Predicted band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11220

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-9 (ANXA9) acts as a calcium/phospholipid-binding protein and belongs to the annexin family characterized by their dependency on calcium for membrane binding. ANXA9 has a molecular mass of about 36 kDa. It expresses in epithelial tissues particularly within the skin esophagus and the major airways suggesting its role in cellular homeostasis and communication in these areas. ANXA9's structure includes typical annexin repeats responsible for its binding properties and it shares similarities with other annexins.

Biological function summary

ANXA9 participates in membrane-related processes such as vesicle trafficking and membrane organization. It engages in forming stable complexes with other annexin proteins enhancing their collective functions involved in cell signaling or membrane dynamics. The protein localizes near cell membranes and aids in mediating cell-cell interactions signifying its involvement in maintaining cellular environments.

Pathways

ANXA9 connects with signaling pathways that are important for phospholipid metabolism and membrane trafficking. It interacts with proteins like ANXA1 and ANXA2 which also belong to the annexin family and perform overlapping functions in modulating cellular responses to extracellular signals. Understanding its precise role in these pathways can provide insights into its influence on cell surface receptor-mediated signaling and lipid-related metabolic processes.

Studies link ANXA9 with certain epithelial cancers given its pattern of expression in epithelial tissues. Its abnormal regulation may associate with tumor growth or progression due to its role in cell membrane stability and signal transduction. Additionally ANXA9 is associated with skin disorders where it might interact with proteins such as filaggrin contributing to barrier dysfunctions or inflammatory responses. These connections highlight the need for further research to elucidate ANXA9’s exact functions and its potential as a therapeutic target in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in epidermal differentiation (PubMed : 10899159). Can bind acetylcholine (PubMed : 10899159).

See full target information ANXA9

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40586312

2025

ERH regulates type II interferon immune signaling through post-transcriptional regulation of JAK2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Soderholm,Milica Vunjak,Melanie de Almeida,Niko Popitsch,Nadezda Podvalnaya,Pablo Araguas-Rodriguez,Sara Scinicariello,Emily Nischwitz,Falk Butter,René F Ketting,Stefan L Ameres,Michaela Müller-McNicoll,Johannes Zuber,Gijs A Versteeg

Cell death & disease 15:260 PubMed38609357

2024

ANXA9 facilitates S100A4 and promotes breast cancer progression through modulating STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiqian Zhou,Junyong Zhao,Tao Yan,Danrong Ye,Yuying Wang,Bai'an Zhou,Diya Liu,Xuehui Wang,Wenfang Zheng,Bowen Zheng,Fengyuan Qian,Yating Li,Dengfeng Li,Lin Fang

Frontiers in oncology 13:1166666 PubMed37841425

2023

miR-186-ANXA9 signaling inhibits tumorigenesis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongrui Wang,Xiqian Zhou,Xiaochong Deng,Danrong Ye,Diya Liu,Baian Zhou,Wenfang Zheng,Xuehui Wang,Yuying Wang,Oyungerel Borkhuu,Lin Fang

The journal of pathology. Clinical research 9:378-390 PubMed37294149

2023

Exosome-derived ANXA9 functions as an oncogene in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cuiping Lu,Ying Zhan,Yunshan Jiang,Jianrong Liao,Zidan Qiu

Translational lung cancer research 12:1062-1077 PubMed37323183

2023

Harmine loaded Au@MSNs@PEG@Asp6 nano-composites for treatment of spinal metastasis from lung adenocarcinoma by targeting ANXA9 experiment.

Applications

Unspecified application

Species

Unspecified reactive species

Houlei Wang,Fancheng Chen,Annan Hu,Haifeng Liang,Yun Liang,Nagashree Seetharamu,Huiren Wang,Jian Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin-9/ANXA9 antibody [EPR11220]

Immunoprecipitation - Anti-Annexin-9/ANXA9 antibody [EPR11220] (AB166621)

Western blot - Anti-Annexin-9/ANXA9 antibody [EPR11220] (AB166621)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise