AB135256

Anti-Annexin A1/ANXA1 antibody [2F1]

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(2 Publications)

Mouse Monoclonal Annexin A1/ANXA1 antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Human, Mouse, Rat, African green monkey samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANXA1 aa 100-250.

View Alternative Names

ANX1, LPC1, ANXA1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.

Lab

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : Annexin A1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HepG2 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab135256 observed at 38 kDa. Red - loading control, ab176560, observed at 50 kDa.

ab135256 was shown to specifically react with Annexin A1 when Annexin A1 knockout samples were used. Wild-type and Annexin A1 knockout samples were subjected to SDS-PAGE. ab135256 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)

Predicted band size: 38 kDa

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Unknown

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Expected MW of recombinant fragment is 33.7 kDa.

All lanes:

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution

All lanes:

Human Annexin A1 recombinant protein.

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

All lanes:

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

A549 cell lysate

Lane 3:

K562 cell lysate

Lane 4:

NIH 3T3 cell lysate

Lane 5:

C6 cell lysate

Lane 6:

COS7 cell lysate

Predicted band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2F1

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ANXA1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P04083

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.

Biological function summary

Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.

Pathways

Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.

Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed : 8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T cells (PubMed : 17008549). Promotes the differentiation of T cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed : 17008549). Has no effect on unstimulated T cells (PubMed : 17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed : 19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed : 2532504, PubMed : 8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). In the context of antitumor immunity, interacts with FPR1 on dendritic cells allowing for tumor-associated antigens uptake and cross-presentation to T cells to mount an antitumor specific T cell response.. Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed : 15187149, PubMed : 22879591, PubMed : 25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed : 15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed : 15187149). Promotes resolution of inflammation and wound healing (PubMed : 25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed : 22879591).

See full target information ANXA1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed36744428

2023

LRRK1 functions as a scaffold for PTP1B-mediated EGFR sorting into ILVs at the ER-endosome contact site.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Hanafusa,Keitaro Fujita,Misa Kamio,Shiori Iida,Yasushi Tamura,Naoki Hisamoto,Kunihiro Matsumoto

Nature communications 13:7522 PubMed36473871

2022

The dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Dall'Agnese,Jesse M Platt,Ming M Zheng,Max Friesen,Giuseppe Dall'Agnese,Alyssa M Blaise,Jessica B Spinelli,Jonathan E Henninger,Erin N Tevonian,Nancy M Hannett,Charalampos Lazaris,Hannah K Drescher,Lea M Bartsch,Henry R Kilgore,Rudolf Jaenisch,Linda G Griffith,Ibrahim I Cisse,Jacob F Jeppesen,Tong I Lee,Richard A Young

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin A1/ANXA1 antibody [2F1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (AB135256)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise