AB137745

Anti-Annexin A1/ANXA1 antibody

Name: Anti-Annexin A1/ANXA1 antibody (ab137745) | Abcam
Brand: Abcam Limited
SKU: AB137745

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(6 Publications)

Rabbit Polyclonal Annexin A1/ANXA1 antibody. Suitable for IHC-P, WB, IP, ICC/IF and reacts with Human, Mouse, Transfected cell lysate samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANXA1 aa 1 to C-terminus.

View Alternative Names

ANX1, LPC1, ANXA1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody (AB137745)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody (AB137745)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Annexin A1 protein using ab137745 at a 1/500 dilution (Green). DAPI was used for nuclear staining. Scale bar = 10 µm

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody (AB137745)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody (AB137745)

Immunohistochemical analysis of paraffin-embedded N87 Xenograft, labelling Annexin A1 using ab137745 at 1/500 dilution.

Supplier Data

Immunoprecipitation - Anti-Annexin A1/ANXA1 antibody (AB137745)

Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 µg of Annexin A1 antibody.
Western blot analysis was performed using Annexin A1 antibody.

All lanes:

Immunoprecipitation - Anti-Annexin A1/ANXA1 antibody (ab137745) at 5 µg

Lane 1:

HeLa whole cell extracts (Input)

Lane 2:

HeLa whole cell extracts with control IgG (-)

Lane 3:

HeLa whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG Antibody

Predicted band size: 39 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody (AB137745)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue stained for Annexin A1 protein at nucleus with ab137745 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/10000 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 38 kDa

false

Supplier Data

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/1000 dilution

Lane 1:

Wild-type Annexin A1 HeLa cell extracts at 30 µg

Lane 2:

Knockout Annexin A1 HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 38 kDa

false

Supplier Data

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/10000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

ANXA1 transfected 293T whole cell extracts at 30 µg

Lane 3:

ANXA2 transfected 293 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 38 kDa

false

Supplier Data

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/10000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 38 kDa

false

Supplier Data

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/10000 dilution

Lane 1:

C6 whole cell extracts at 30 µg

Lane 2:

NIH-3T3 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ANXA1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04083

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.

Biological function summary

Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.

Pathways

Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.

Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed : 8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed : 17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed : 17008549). Has no effect on unstimulated T cells (PubMed : 17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed : 19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed : 2532504, PubMed : 8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).. Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed : 15187149, PubMed : 22879591, PubMed : 25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed : 15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed : 15187149). Promotes resolution of inflammation and wound healing (PubMed : 25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed : 22879591).

See full target information ANXA1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Aging and disease 16:3567-3583 PubMed39751866

2025

Aerobic Exercise Improves Cognitive Recovery in Mice with Chronic Cerebral Hypoperfusion by Modulating the Annexin-A1-MAPK Axis and Astrocyte Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Jing He,Yuxin Wang,Xiaozhen Wang,He Jin,Xu Zhang,Ling Kong,Yanchuan Wu,Yong Yang,Rong Wang

Brain sciences 14: PubMed38790419

2024

Annexin 1 Reduces Dermatitis-Induced Itch and Cholestatic Itch through Inhibiting Neuroinflammation and Iron Overload in the Spinal Dorsal Horn of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tang Li,Lingyue Hu,Chao Qin,Yuanjie Li,Zhenhua Song,Yang Jiao,Chunyan Wang,Wei Cui,Linlin Zhang

International journal of molecular sciences 24: PubMed36983004

2023

Time Dependent Changes in the Ovine Neurovascular Unit; A Potential Neuroprotective Role of Annexin A1 in Neonatal Hypoxic-Ischemic Encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Young Park,Valéry L E van Bruggen,Carine J Peutz-Kootstra,Daan R M G Ophelders,Reint K Jellema,Chris P M Reutelingsperger,Bart P F Rutten,Tim G A M Wolfs

Brain, behavior, & immunity - health 23:100458 PubMed35647567

2022

Prenatal administration of multipotent adult progenitor cells modulates the systemic and cerebral immune response in an ovine model of chorioamnionitis.

Applications

Unspecified application

Species

Unspecified reactive species

Luise Klein,Daan R M G Ophelders,Daniel van den Hove,Maurits Damoiseaux,Bart P F Rutten,Chris P M Reutelingsperger,Leon J Schurgers,Tim G A M Wolfs

International journal of oncology 57:1203-1213 PubMed32901832

2020

ANXA1‑derived peptides suppress gastric and colon cancer cell growth by targeting EphA2 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Feng,Ta Xiao,Shan-Shan Lu,Xiao-Pu Hung,Hong Yi,Qiu-Yan He,Wei Huang,Yao-Yun Tang,Zhi-Qiang Xiao

Molecular medicine reports 13:3858-66 PubMed27035116

2016

Knockdown of ANXA1 suppresses the biological behavior of human NSCLC cells in vitro.

Applications

Species

Human

Ying Fang,Xiaoying Guan,Tonghui Cai,Jie Long,Hongyan Wang,Xiaobin Xie,Yajie Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Annexin A1/ANXA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody (AB137745)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody (AB137745)

Immunoprecipitation - Anti-Annexin A1/ANXA1 antibody (AB137745)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody (AB137745)

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Western blot - Anti-Annexin A1/ANXA1 antibody (AB137745)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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