Mouse Monoclonal Annexin A1/ANXA1 antibody. Carrier free. Suitable for Flow Cyt, WB, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANXA1.
Images
![Flow Cytometry - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--flow-cytometry-img74874.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img39740.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)")
![Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunocytochemistry-immunofluorescence-img118732.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)")
![Western blot - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--western-blot-img45448.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91392.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ANXA1. Database link P04083
Reactivity data
Select an application| Flow Cyt | WB | Protein Array | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:22879591, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591).
Alternative names
ANX1, LPC1, ANXA1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35
Recommended products
Mouse Monoclonal Annexin A1/ANXA1 antibody. Carrier free. Suitable for Flow Cyt, WB, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANXA1.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ANXA1. Database link P04083
- Carrier free
- Yes
- Clone number
- ANXA1/3566
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab268191 is the carrier-free version of Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.
- Biological function summary
Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.
- Pathways
Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.
- Associated diseases and disorders
Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Flow Cytometry - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--flow-cytometry-img74874.jpg "Flow Cytometry - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Flow Cytometry - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)
Flow cytometric analysis of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin I using Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img39740.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Annexin I using Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070)
![Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunocytochemistry-immunofluorescence-img118732.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)
Immunofluorescence staining of paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Annexin I with Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070 at 2 μg/ml followed by a goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070)
![Western blot - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--western-blot-img45448.jpg "Western blot - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Western blot - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070)
All lanes: Western blot - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070) at 2 µg/mL
All lanes: A549 (Human lung carcinoma cell line) whole cell lysate
Predicted band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91392.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Annexin I using Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61971.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for Annexin I using Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40274.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for Annexin I using Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070)
![Protein Array - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--protein-array-img141307.jpg "Protein Array - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Protein Array - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)
Protein Array containing more than 19,000 full-length human proteins using Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] ab268070)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61971.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40274.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
![Protein Array - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191), expandable thumbnail](https://content.abcam.com/products/images/annexin-a1-anxa1-antibody-anxa1-3566-bsa-and-azide-free-ab268191--protein-array-img141307.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (ab268191)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com