Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free
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(1 Publication)
Mouse Monoclonal Annexin A1/ANXA1 antibody. Carrier free. Suitable for Flow Cyt, WB, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANXA1.
View Alternative Names
ANX1, LPC1, ANXA1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for Annexin I using ab268070 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)
Immunofluorescence staining of paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Annexin I with ab268070 at 2 μg/ml followed by a goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Annexin I using ab268070 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for Annexin I using ab268070 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
- Flow Cyt
Unknown
Flow Cytometry - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)
Flow cytometric analysis of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin I using ab268070 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Annexin I using ab268070 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
- WB
Unknown
Western blot - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
All lanes:
Western blot - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] (<a href='/en-us/products/primary-antibodies/annexin-a1-anxa1-antibody-anxa1-3566-ab268070'>ab268070</a>) at 2 µg/mL
All lanes:
A549 (Human lung carcinoma cell line) whole cell lysate
Predicted band size: 38 kDa
false
- Protein Array
Unknown
Protein Array - Anti-Annexin A1/ANXA1 antibody [ANXA1/3566] - BSA and Azide free (AB268191)
Protein Array containing more than 19,000 full-length human proteins using ab268070.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268070)
Reactivity data
Product details
ab268191 is the carrier-free version of ab268070.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.
Pathways
Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.
Product protocols
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Journal for immunotherapy of cancer 11: PubMed37072348
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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