AB127924

Anti-Annexin A3/ANXA3 antibody

Name: Anti-Annexin A3/ANXA3 antibody (ab127924) | Abcam
Brand: Abcam Limited
SKU: AB127924
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal Annexin A3/ANXA3 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Rat Anxa3 aa 100-150.

View Alternative Names

ANX3, ANXA3, Annexin A3, 35-alpha calcimedin, Annexin III, Annexin-3, Lipocortin III, Placental anticoagulant protein III, PAP-III

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody (AB127924)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody (AB127924)

ANXA3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab127924 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody (AB127924)

ANXA3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab127924 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Rat Anxa3 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P14669

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.31% Thimerosal (merthiolate), 0.06% Sodium azide Constituents: 30.75% BSA, 5.54% Sodium chloride, 1.23% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A3 also known as ANXA3 is a 36 kDa protein that belongs to the annexin family. This family is known for calcium-dependent phospholipid-binding activity. ANXA3 specifically binds to phosphatidylserine a type of phospholipid found in cell membranes. It is expressed in various tissues with notable presence in myeloid cells particularly in the bone marrow and granulocytes.

Biological function summary

ANXA3 plays roles in membrane-related events including vesicle trafficking and exocytosis. It is not part of a large complex but associates with other proteins depending on its cellular function. ANXA3 can also regulate inflammation by modulating macrophage activation and may influence signal transduction pathways that are linked to immune responses.

Pathways

ANXA3 functions in both inflammation and apoptosis. It is closely related to the apoptosis pathway by interacting with signaling molecules like caspases which are critical for the execution of cell death. Additionally through its action in the inflammation pathway ANXA3 is known to interact with proteins such as NF-κB an important regulator of immune and inflammatory responses.

Alterations in ANXA3 expression or function associate with various conditions most notably cancer and autoimmune diseases. For instance in certain cancers ANXA3 may contribute to tumor growth and metastasis. In autoimmune diseases its regulation of macrophage activity can misbalance immune responses leading to pathogenesis. Proteins such as ANXA1 and S100A11 also play roles in these pathways and may function along with ANXA3 in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.

See full target information ANXA3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107807 PubMed39307302

2024

Aberrant overexpression of myosin 1b in glioblastoma promotes angiogenesis via VEGF-myc-myosin 1b-Piezo1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Lv,Fan Yang,Zhengmao Ge,Lele Xin,Lingxue Zhang,Yaohong Zhai,Xian Liu,Qingdong Guo,Xinggang Mao,Peng Luo,Lei Zhang,Xiaofan Jiang,Yanyu Zhang

Cell death discovery 9:101 PubMed36944636

2023

The long non-coding RNA PVT1 promotes tumorigenesis of cutaneous squamous cell carcinoma via interaction with 4EBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Dan Huang,Mei Ju,Hong-Ying Chen,Chao Luan,Jia-An Zhang,Kun Chen

Molecular therapy. Nucleic acids 17:540-550 PubMed31362241

2019

ANXA3 Silencing Ameliorates Intracranial Aneurysm via Inhibition of the JNK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Chun Wang,Qi Yang,Yan-Li Cheng

BioMed research international 2017:2603685 PubMed28497041

2017

Annexin A3 as a Prognostic Biomarker for Breast Cancer: A Retrospective Study.

Applications

IHC

Species

Human

Tao Zhou,Yong Li,Li Yang,Tiantian Tang,Lina Zhang,Jiajie Shi

Journal of neurochemistry 134:200-10 PubMed25832806

2015

Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels.

Applications

Unspecified application

Species

Unspecified reactive species

Margaret E Tome,Charles P Schaefer,Leigh M Jacobs,Yifeng Zhang,Joseph M Herndon,Fabian O Matty,Thomas P Davis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin A3/ANXA3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody (AB127924)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody (AB127924)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise