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AB273649

Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free

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Mouse Monoclonal Annexin A3/ANXA3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, African green monkey, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANXA3.

View Alternative Names

ANX3, ANXA3, Annexin A3, 35-alpha calcimedin, Annexin III, Annexin-3, Lipocortin III, Placental anticoagulant protein III, PAP-III

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (AB273649)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (AB273649)

Immunohistochemical analysis of paraffin-embedded human prostate tissue staining of ANXA3 with a mouse monoclonal
Annexin A3/ANXA3 antibody [OTI5G4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (AB273649)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (AB273649)

Immunohistochemical analysis of paraffin-embedded human lung tissue staining of ANXA3 with a mouse monoclonal
Annexin A3/ANXA3 antibody [OTI5G4]

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (AB273649)
  • WB

Supplier Data

Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (AB273649)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

All lanes:

Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY ANXA3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

Predicted band size: 36 kDa

false

Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (AB273649)
  • WB

Supplier Data

Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (AB273649)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

All lanes:

Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649) at 1/2000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

Lane 7:

MDCK (canine kidney cell line) whole cell lysate at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI5G4

Isotype

IgG1

Carrier free

Yes

Reacts with

Dog, Human, African green monkey

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ANXA3.

P12429

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.3 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A3 also known as ANXA3 is a 36 kDa protein that belongs to the annexin family. This family is known for calcium-dependent phospholipid-binding activity. ANXA3 specifically binds to phosphatidylserine a type of phospholipid found in cell membranes. It is expressed in various tissues with notable presence in myeloid cells particularly in the bone marrow and granulocytes.
Biological function summary

ANXA3 plays roles in membrane-related events including vesicle trafficking and exocytosis. It is not part of a large complex but associates with other proteins depending on its cellular function. ANXA3 can also regulate inflammation by modulating macrophage activation and may influence signal transduction pathways that are linked to immune responses.

Pathways

ANXA3 functions in both inflammation and apoptosis. It is closely related to the apoptosis pathway by interacting with signaling molecules like caspases which are critical for the execution of cell death. Additionally through its action in the inflammation pathway ANXA3 is known to interact with proteins such as NF-κB an important regulator of immune and inflammatory responses.

Alterations in ANXA3 expression or function associate with various conditions most notably cancer and autoimmune diseases. For instance in certain cancers ANXA3 may contribute to tumor growth and metastasis. In autoimmune diseases its regulation of macrophage activity can misbalance immune responses leading to pathogenesis. Proteins such as ANXA1 and S100A11 also play roles in these pathways and may function along with ANXA3 in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
See full target information ANXA3
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Product promise

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