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Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775) is a mouse monoclonal antibody that is used to detect Annexin V/ANXA5 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human samples.



- Over 20 publications

- Trusted since 2007


Images

Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (AB54775), expandable thumbnail
  • Flow Cytometry - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (AB54775), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (AB54775), expandable thumbnail
  • Immunoprecipitation - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (AB54775), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (AB54775), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human ANXA5. Database link P08758

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

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Target data

Function

The protein expressed by gene ANXA5 is an anticoagulant protein that indirectly inhibits the thromboplastin-specific complex, a component involved in the blood coagulation cascade. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha

Recommended products

Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775) is a mouse monoclonal antibody that is used to detect Annexin V/ANXA5 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human samples.



- Over 20 publications

- Trusted since 2007

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human ANXA5. Database link P08758
Carrier free
Yes
Clone number
1F4-1A5
Purity
Tissue culture supernatant
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Annexin V/ANXA5?


Anti-Annexin V/ANXA5 [1F4-1A5] (ab54775) specifically detects a band for Annexin V/ANXA5 (UniProt: P08758) at a molecular weight of 36kDa.

Trusted by the scientific community


Anti-Annexin V/ANXA5 [1F4-1A5] (ab54775) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.

Reviewed by scientists


Anti-Annexin V/ANXA5 [1F4-1A5] (ab54775) has over 5 independent reviews from customers.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.

Biological function summary

Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.

Pathways

Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.

Associated diseases and disorders

Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.

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7 product images

  • Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775), expandable thumbnail

    Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    ab54775 Western Blot analysis of ANXA5/ANXA5 expression in Hela cells.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    Predicted band size: 35 kDa

  • Flow Cytometry - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775), expandable thumbnail

    Flow Cytometry - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    Overlay histogram showing HeLa cells stained with ab54775 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54775, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    Annexin V/ANXA5 antibody (ab54775) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.

    This image was generated using the ascites version of the product.

  • Immunoprecipitation - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775), expandable thumbnail

    Immunoprecipitation - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    Immunoprecipitation of ANXA5 transfected lysate using ab54775 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with a ANXA5 rabbit polyclonal antibody.

    This image was generated using the ascites version of the product.

    All lanes: Immunoprecipitation - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    Predicted band size: 35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    ab54775 at 10 ug/ml staining Annexin V/ANXA5 in human Hela cells by Immunocytochemistry / Immunofluorescence.

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    ab54775 staining Annexin V/ANXA5 in HeLa cells treated with pentoxifylline (Pentoxifylline, Phosphodiesterase and cytokine inhibitor ab120725), by ICC/IF. Increase of Annexin V/ANXA5 correlates with increased concentration of pentoxifylline, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of Pentoxifylline, Phosphodiesterase and cytokine inhibitor ab120725 (pentoxifylline) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab54775 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775), expandable thumbnail

    Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    Western Blot analysis of ANXA5 expression in transfected 293T cell line by ab54775.
    Lane 1: ANXA5 transfected lysate(35.9 KDa).
    Lane 2: Non-transfected lysate.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

    Predicted band size: 35 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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