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Rabbit Polyclonal Annexin V/ANXA5 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Annexin A5 aa 50-300.

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Images

Flow Cytometry - Anti-Annexin V/ANXA5 antibody (AB137823), expandable thumbnail
  • Western blot - Anti-Annexin V/ANXA5 antibody (AB137823), expandable thumbnail
  • Western blot - Anti-Annexin V/ANXA5 antibody (AB137823), expandable thumbnail
  • Western blot - Anti-Annexin V/ANXA5 antibody (AB137823), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Annexin A5 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link P08758

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50 - 1/200

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Alternative names

Recommended products

Rabbit Polyclonal Annexin V/ANXA5 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Annexin A5 aa 50-300.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Annexin A5 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link P08758
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.

Biological function summary

Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.

Pathways

Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.

Associated diseases and disorders

Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Flow Cytometry - Anti-Annexin V/ANXA5 antibody (ab137823), expandable thumbnail

    Flow Cytometry - Anti-Annexin V/ANXA5 antibody (ab137823)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin V/ANXA5 using ab137823 at 1/50 dilution (red) compared to an unlabeled control (black).

    A Dylight 488 secondary antibody was used.

  • Western blot - Anti-Annexin V/ANXA5 antibody (ab137823), expandable thumbnail

    Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)

    All lanes: Western blot - Anti-Annexin V/ANXA5 antibody (ab137823) at 1/5000 dilution

    Lane 1: WT 293T cell extracts at 30 µg

    Lane 2: Annexin V/ANXA5 Knockout 293T cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG

    Predicted band size: 35 kDa, 54 kDa, 69 kDa

  • Western blot - Anti-Annexin V/ANXA5 antibody (ab137823), expandable thumbnail

    Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)

    10% SDS PAGE

    All lanes: Western blot - Anti-Annexin V/ANXA5 antibody (ab137823) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 30 µg

    Lane 2: HepG2 whole cell lysate at 30 µg

    Predicted band size: 35 kDa

  • Western blot - Anti-Annexin V/ANXA5 antibody (ab137823), expandable thumbnail

    Western blot - Anti-Annexin V/ANXA5 antibody (ab137823)

    10% SDS PAGE

    All lanes: Western blot - Anti-Annexin V/ANXA5 antibody (ab137823) at 1/1000 dilution

    All lanes: Mouse brain whole cell lysate at 50 µg

    Predicted band size: 35 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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