AB140068

Anti-Annexin V/ANXA5 antibody

Name: Anti-Annexin V/ANXA5 antibody (ab140068) | Abcam
Brand: Abcam Limited
SKU: AB140068
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Annexin V/ANXA5 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANXA5.

View Alternative Names

ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha

6 Images

Flow Cytometry - Anti-Annexin V/ANXA5 antibody (AB140068)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Annexin V/ANXA5 antibody (AB140068)

Flow cytometry analysis of HeLa cells labelling Annexin V protein with ab140068 at a 1/50 dilution compared with unlabelled control. Acquisition of 20,000 events were collected for FACS.

Supplier Data

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-Annexin V/ANXA5 antibody (ab140068) at 1/5000 dilution

Lane 1:

293T whole cell extract at 30 µg

Lane 2:

HepG2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

All lanes:

Western blot - Anti-Annexin V/ANXA5 antibody (ab140068) at 1/5000 dilution

Lane 1:

GL261 whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

All lanes:

Western blot - Anti-Annexin V/ANXA5 antibody (ab140068) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

All lanes:

Western blot - Anti-Annexin V/ANXA5 antibody (ab140068) at 1/5000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Lab

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

All lanes:

Western blot - Anti-Annexin V/ANXA5 antibody (ab140068) at 1/50000 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

Annexin V/ANXA5 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ANXA5.

P08758

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/50000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/50000", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/50000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.

Biological function summary

Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.

Pathways

Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.

Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene ANXA5 is an anticoagulant protein that indirectly inhibits the thromboplastin-specific complex, a component involved in the blood coagulation cascade. This supplementary information is collated from multiple sources and compiled automatically.

See full target information ANXA5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:846 PubMed37582968

2023

Sexual dimorphism in the social behaviour of Cntnap2-null mice correlates with disrupted synaptic connectivity and increased microglial activity in the anterior cingulate cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Matt S Dawson,Kevin Gordon-Fleet,Lingxin Yan,Vera Tardos,Huanying He,Kwong Mui,Smriti Nawani,Zeinab Asgarian,Marco Catani,Cathy Fernandes,Uwe Drescher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin V/ANXA5 antibody

Flow Cytometry - Anti-Annexin V/ANXA5 antibody (AB140068)

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

Western blot - Anti-Annexin V/ANXA5 antibody (AB140068)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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