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Rabbit Recombinant Monoclonal Annexin V/ANXA5 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (AB239922), expandable thumbnail
  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (AB239922), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (AB239922), expandable thumbnail
  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (AB239922), expandable thumbnail
  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (AB239922), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Alternative names

Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CBP-I, PP4, PAP-I, VAC-alpha, ANXA5, ANX5, ENX2, PP4

Recommended products

Rabbit Recombinant Monoclonal Annexin V/ANXA5 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3980

Purification technique

Affinity purification Protein A

Specificity

The immunogen used for this product shares 80% homology with ANXA2 and 73% homology with ANXA4. Cross-reactivity with this protein has not been confirmed experimentally.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab239922 is the carrier-free version of Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.

Biological function summary

Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.

Pathways

Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.

Associated diseases and disorders

Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)

    This data was developed using Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Annexin V/ANXA5 with purified Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922), expandable thumbnail

    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)

    This data was developed using Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Annexin V knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)

    Lanes 1 - 3: Merged signal (red and green). Green - Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

    Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194 was shown to recognize Annexin V in wild-type cells as signal was lost at the expected MW in Annexin V knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Annexin V knockout samples were subjected to SDS-PAGE. Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)

    Predicted band size: 35 kDa

  • Flow Cytometry (Intracellular) - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)

    This data was developed using Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Annexin V/ANXA5 with purified Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194 at 1/30 dilution (10 µg/ml) (red). Cells were fixed with 80% Methanol and permeabilised with 0.1% Tween-20. A Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).

  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922), expandable thumbnail

    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)

    This data was developed using Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 3: Mouse brain at 20 µg

    Lane 4: Mouse heart at 20 µg

    Lane 5: Rat brain at 20 µg

    Lane 6: Rat heart at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922), expandable thumbnail

    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] - BSA and Azide free (ab239922)

    This data was developed using Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194, the same antibody clone in a different buffer formulation.

    Western blot: Anti-ANXA5 antibody [EPR3980] (Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194) staining at 1/10000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194 was shown to bind specifically to ANXA5. A band was observed at 36 kDa in wild-type A549 cell lysates with no signal observed at this size in ANXA5 knockout cell line. To generate this image, wild-type and ANXA5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Annexin V/ANXA5 antibody [EPR3980] (Anti-Annexin V/ANXA5 antibody [EPR3980] ab108194) at 1/10000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: ANXA5 knockout A549 cell lysate at 20 µg

    Secondary

    Lanes 1 - 2: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 2: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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