AB234422

Anti-ANO6 antibody [EPR20910-105]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal ANO6 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Tmem16f, Ano6, Anoctamin-6, Small-conductance calcium-activated nonselective cation channel, Transmembrane protein 16F, SCAN channel

2 Images

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Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] (AB234422)

ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1 : Mouse spleen lysate 10 μg (Input).
Lane 2 : ab234422 IP in mouse spleen lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 3 mins.

The molecular weight observed is consistent with literatures (PMID : 29507235, PMID : 26417084, PMID : 27227820, PMID : 24478309)

All lanes:

Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] (ab234422)

Predicted band size: 106 kDa

Observed band size: 100-250 kDa,120-250 kDa

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Western blot - Anti-ANO6 antibody [EPR20910-105] (AB234422)

The molecular weight observed is consistent with literatures (PMID : 29507235, PMID : 26417084, PMID : 27227820, PMID : 24478309).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 37 secs; Lane 3 : 124 secs; Lane 4 : 70 secs.

All lanes:

Western blot - Anti-ANO6 antibody [EPR20910-105] (ab234422) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 106 kDa

Observed band size: 120-250 kDa

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Carrier free

Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20910-105

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anoctamin 6 also known as ANO6 TMEM16F or NGEP is a protein that functions mechanically as a calcium-activated chloride channel and scramblase. It plays a role in phospholipid scrambling across the plasma membrane influencing membrane stability and shape. ANO6 has a mass of approximately 114 kDa. Researchers have detected ANO6 in a variety of tissues with higher expression noted in the heart skeletal muscles and testis.

Biological function summary

ANO6 is involved in critical cellular processes such as apoptosis and blood coagulation. This protein contributes to the externalization of phosphatidylserine a process necessary during cell death and platelet activation. ANO6 does not strictly function alone; it often interacts with other members of the TMEM16 family forming complexes that modulate its functional specificity and activity.

Pathways

ANO6 is integral to the extrinsic pathway of apoptosis and the process of blood coagulation. It plays a role in regulating cell membrane ion homeostasis working alongside related proteins such as ANO1 and ANO2. These pathways maintain the stability and balance of cellular environments influencing processes like cell shrinkage and coagulative events.

ANO6 relates to conditions like Scott syndrome a bleeding disorder characterized by defective phosphatidylserine exposure on platelets and can impact certain cardiac arrhythmias. ANO6's role in these disorders often involves interaction with other regulatory proteins such as phospholipase C which impacts phospholipid signaling and platelet function. Understanding ANO6's involvement in these processes aids in developing potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.

See full target information Ano6

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadx6587 PubMed40815641

2025

Targeting lipid scrambling potentiates ferroptosis and triggers tumor immune rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyun Yang,Ze Yu,Jieming Ping,Yuanchun Duan,Jianlong Tang,Weixiang Liu,Qing He,Yongfeng Lai,Sin Man Lam,Hesen Tang,Zhengjie Liu,Weimin Wang,Min Zhu,Wei Hu,Yunyun Han,Guanghou Shui,Jiqing Hao,Zheng Liu,Ning Wu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402693 PubMed39136057

2024

TMEM16F Expressed in Kupffer Cells Regulates Liver Inflammation and Metabolism to Protect Against Listeria Monocytogenes.

Applications

Unspecified application

Species

Unspecified reactive species

Jianlong Tang,Hua Song,Shimin Li,Sin Man Lam,Jieming Ping,Mengyun Yang,Na Li,Teding Chang,Ze Yu,Weixiang Liu,Yan Lu,Min Zhu,Zhaohui Tang,Zheng Liu,Yusong R Guo,Guanghou Shui,André Veillette,Zhutian Zeng,Ning Wu

Molecular cell 81:3526-3541.e8 PubMed34186021

2021

BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Conway,Federico Rossi,Daniel Fernandez-Perez,Eleonora Ponzo,Karin Johanna Ferrari,Marika Zanotti,Daria Manganaro,Simona Rodighiero,Simone Tamburri,Diego Pasini

Nature 594:88-93 PubMed33827113

2021

Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Braga,Hashim Ali,Ilaria Secco,Elena Chiavacci,Guilherme Neves,Daniel Goldhill,Rebecca Penn,Jose M Jimenez-Guardeño,Ana M Ortega-Prieto,Rossana Bussani,Antonio Cannatà,Giorgia Rizzari,Chiara Collesi,Edoardo Schneider,Daniele Arosio,Ajay M Shah,Wendy S Barclay,Michael H Malim,Juan Burrone,Mauro Giacca

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ANO6 antibody [EPR20910-105]

Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] (AB234422)

Western blot - Anti-ANO6 antibody [EPR20910-105] (AB234422)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise