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AB256302

Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal ANO6 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Tmem16f, Ano6, Anoctamin-6, Small-conductance calcium-activated nonselective cation channel, Transmembrane protein 16F, SCAN channel

1 Images
Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (AB256302)
  • IP

Unknown

Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] - BSA and Azide free (AB256302)

ANO6 was immunoprecipitated from 0.35 mg mouse spleen lysate with ab234422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234422 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1 : Mouse spleen lysate 10 μg (Input).
Lane 2 : ab234422 IP in mouse spleen lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab234422 in mouse spleen lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 3 mins.

The molecular weight observed is consistent with literatures (PMID : 29507235, PMID : 26417084, PMID : 27227820, PMID : 24478309)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234422).

All lanes:

Immunoprecipitation - Anti-ANO6 antibody [EPR20910-105] (<a href='/en-us/products/primary-antibodies/ano6-antibody-epr20910-105-ab234422'>ab234422</a>)

Predicted band size: 106 kDa

Observed band size: 100-250 kDa,120-250 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20910-105

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab256302 is the carrier-free version of ab234422.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anoctamin 6 also known as ANO6 TMEM16F or NGEP is a protein that functions mechanically as a calcium-activated chloride channel and scramblase. It plays a role in phospholipid scrambling across the plasma membrane influencing membrane stability and shape. ANO6 has a mass of approximately 114 kDa. Researchers have detected ANO6 in a variety of tissues with higher expression noted in the heart skeletal muscles and testis.
Biological function summary

ANO6 is involved in critical cellular processes such as apoptosis and blood coagulation. This protein contributes to the externalization of phosphatidylserine a process necessary during cell death and platelet activation. ANO6 does not strictly function alone; it often interacts with other members of the TMEM16 family forming complexes that modulate its functional specificity and activity.

Pathways

ANO6 is integral to the extrinsic pathway of apoptosis and the process of blood coagulation. It plays a role in regulating cell membrane ion homeostasis working alongside related proteins such as ANO1 and ANO2. These pathways maintain the stability and balance of cellular environments influencing processes like cell shrinkage and coagulative events.

ANO6 relates to conditions like Scott syndrome a bleeding disorder characterized by defective phosphatidylserine exposure on platelets and can impact certain cardiac arrhythmias. ANO6's role in these disorders often involves interaction with other regulatory proteins such as phospholipase C which impacts phospholipid signaling and platelet function. Understanding ANO6's involvement in these processes aids in developing potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.
See full target information Ano6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:573 PubMed39117605

2024

Elucidating the role of S100A10 in CD8 T cell exhaustion and HCC immune escape via the cPLA2 and 5-LOX axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ganggang Wang,Xiaowei Shen,Wenzhi Jin,Chao Song,Meiyuan Dong,Zhijie Zhou,Xiaoliang Wang

Frontiers in cardiovascular medicine 9:1013262 PubMed36684586

2023

SARS-CoV-2 Spike protein activates TMEM16F-mediated platelet procoagulant activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ambra Cappelletto,Harriet E Allan,Marilena Crescente,Edoardo Schneider,Rossana Bussani,Hashim Ali,Ilaria Secco,Simone Vodret,Roberto Simeone,Luca Mascaretti,Serena Zacchigna,Timothy D Warner,Mauro Giacca
View all publications
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Product promise

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