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AB140087

Anti-ANO9 antibody

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(2 Publications)

Rabbit Polyclonal ANO9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANO9 aa 50-250.

View Alternative Names

PIG5, TMEM16J, TP53I5, ANO9, Anoctamin-9, Transmembrane protein 16J, Tumor protein p53-inducible protein 5, p53-induced gene 5 protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANO9 antibody (AB140087)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANO9 antibody (AB140087)

Immunohistochemical analysis of paraffin embedded Human breat carcinoma tissue labelling ANO9 with ab140087 at 1/100 dilution

Western blot - Anti-ANO9 antibody (AB140087)
  • WB

Unknown

Western blot - Anti-ANO9 antibody (AB140087)

All lanes:

Western blot - Anti-ANO9 antibody (ab140087) at 1/1000 dilution

All lanes:

Human fetal brain lysate

Predicted band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ANO9 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

A1A5B4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in 200 µL of water
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANO9 also known as anoctamin-9 or anion channel homolog-9 is a transmembrane protein belonging to the anoctamin family. This protein serves as a calcium-activated chloride channel regulating ion transport across cellular membranes. ANO9 has an approximate molecular mass of 122 kDa. It is expressed in several tissues with notable presence in the colon rectum and lungs suggesting roles that might be linked to these areas in physiological conditions.
Biological function summary

ANO9 participates in electrolyte balance and volume regulation by modulating ion homeostasis. It functions within the cellular membranes to maintain proper ion flux impacting cellular excitability and other vital cellular functions. ANO9 does not typically operate as an isolated unit; its activities often involve interactions with lipid membranes and other proteins facilitating varied cellular responses to physiological stimuli.

Pathways

ANO9 plays a significant role within the calcium signaling pathway where it contributes to the regulation of intracellular calcium levels. This function associates it with proteins like calmodulin and other calcium-binding proteins which help mediate the response to calcium influx. Additionally ANO9 engages in the phospholipid signaling pathway further influencing its interaction with cellular signaling partners.

ANO9 has been implicated in colorectal cancer where its dysregulation leads to altered cell proliferation and survival. It is also associated with cystic fibrosis due to its role in ion channel function which affects mucus secretion and airway surface liquid regulation. In these contexts ANO9 interacts with proteins like CFTR highlighting its involvement in complex disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

PKA-activated nonselective cation channel (PubMed : 29604966). Discriminates poorly among cations but is more permeable to Ca(2+) ions than to monovalent cations (By similarity). Acts as a calcium-activated calcium permeable channel which may operate as a endoplasmic reticulum (ER) Ca(2+)-leak channel, reducing the loading of the ER Ca(2+) store (PubMed : 36520003). Regulates intracellular Ca2+ signals, ion channel activity, and cytokine release in the renal tissue (PubMed : 36520003). Plays an important role in olfaction, amplifying cAMP-evoked cyclic nucleotide-gated (CNG) channel currents in the olfactory sensory neurons (By similarity). Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed : 22178883). Can inhibit the activity of ANO1 (PubMed : 20056604, PubMed : 22946059).
See full target information ANO9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

In vivo (Athens, Greece) 38:2455-2463 PubMed39187367

2024

Cytoplasmic Expression of the EGFL6 Protein Is an Independent Prognostic Factor for Shortened Patient Survival in Human Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Ting Hsu,Yueh-Min Lin,Ming-Tai Hsing,Kun-Tu Yeh,Jeng-Wei Lu,Shun-Fa Yang

Cancer science 112:1026-1037 PubMed33404124

2021

ANO9 regulates PD-L2 expression and binding ability to PD-1 in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Keita Katsurahara,Atsushi Shiozaki,Toshiyuki Kosuga,Hiroki Shimizu,Michihiro Kudou,Tomohiro Arita,Hirotaka Konishi,Shuhei Komatsu,Takeshi Kubota,Hitoshi Fujiwara,Kazuma Okamoto,Mitsuo Kishimoto,Eiichi Konishi,Eigo Otsuji
View all publications
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Product promise

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