Rabbit Polyclonal ANO9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANO9 aa 50-250.
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Publications
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- Cancer science 112:1026-10372021ANO9 regulates PD-L2 expression and binding ability to PD-1 in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeita Katsurahara,Atsushi Shiozaki,Toshiyuki Kosuga,Hiroki Shimizu,Michihiro Kudou,Tomohiro Arita,Hirotaka Konishi,Shuhei Komatsu,Takeshi Kubota,Hitoshi Fujiwara,Kazuma Okamoto,Mitsuo Kishimoto,Eiichi Konishi,Eigo OtsujiPubMed 33404124
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ANO9 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link A1A5B4
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
PKA-activated nonselective cation channel (PubMed:29604966). Discriminates poorly among cations but is more permeable to Ca(2+) ions than to monovalent cations (By similarity). Acts as a calcium-activated calcium permeable channel which may operate as a endoplasmic reticulum (ER) Ca(2+)-leak channel, reducing the loading of the ER Ca(2+) store (PubMed:36520003). Regulates intracellular Ca2+ signals, ion channel activity, and cytokine release in the renal tissue (PubMed:36520003). Plays an important role in olfaction, amplifying cAMP-evoked cyclic nucleotide-gated (CNG) channel currents in the olfactory sensory neurons (By similarity). Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed:22178883). Can inhibit the activity of ANO1 (PubMed:20056604, PubMed:22946059).
Alternative names
PIG5, TMEM16J, TP53I5, ANO9, Anoctamin-9, Transmembrane protein 16J, Tumor protein p53-inducible protein 5, p53-induced gene 5 protein
Recommended products
Rabbit Polyclonal ANO9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANO9 aa 50-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ANO9 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link A1A5B4
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Reconstitute in 200 µL of water
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ANO9 also known as anoctamin-9 or anion channel homolog-9 is a transmembrane protein belonging to the anoctamin family. This protein serves as a calcium-activated chloride channel regulating ion transport across cellular membranes. ANO9 has an approximate molecular mass of 122 kDa. It is expressed in several tissues with notable presence in the colon rectum and lungs suggesting roles that might be linked to these areas in physiological conditions.
- Biological function summary
ANO9 participates in electrolyte balance and volume regulation by modulating ion homeostasis. It functions within the cellular membranes to maintain proper ion flux impacting cellular excitability and other vital cellular functions. ANO9 does not typically operate as an isolated unit; its activities often involve interactions with lipid membranes and other proteins facilitating varied cellular responses to physiological stimuli.
- Pathways
ANO9 plays a significant role within the calcium signaling pathway where it contributes to the regulation of intracellular calcium levels. This function associates it with proteins like calmodulin and other calcium-binding proteins which help mediate the response to calcium influx. Additionally ANO9 engages in the phospholipid signaling pathway further influencing its interaction with cellular signaling partners.
- Associated diseases and disorders
ANO9 has been implicated in colorectal cancer where its dysregulation leads to altered cell proliferation and survival. It is also associated with cystic fibrosis due to its role in ion channel function which affects mucus secretion and airway surface liquid regulation. In these contexts ANO9 interacts with proteins like CFTR highlighting its involvement in complex disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ANO9 antibody (ab140087)
All lanes: Western blot - Anti-ANO9 antibody (ab140087) at 1/1000 dilution
All lanes: Human fetal brain lysate
Predicted band size: 90 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANO9 antibody (ab140087)
Immunohistochemical analysis of paraffin embedded Human breat carcinoma tissue labelling ANO9 with ab140087
at 1/100 dilution
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