Rabbit Polyclonal Anoctamin 7 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ANO7.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ANO7. The exact immunogen used to generate this antibody is proprietary information. Database link Q6IWH7
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Target data
Function
Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed:22075693). May play a role in cell-cell interactions (PubMed:17308099).
Alternative names
NGEP, PCANAP5, TMEM16G, ANO7, Anoctamin-7, Dresden transmembrane protein of the prostate, IPCA-5, New gene expressed in prostate, Prostate cancer-associated protein 5, Transmembrane protein 16G, D-TMPP
Recommended products
Rabbit Polyclonal Anoctamin 7 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ANO7.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ANO7. The exact immunogen used to generate this antibody is proprietary information. Database link Q6IWH7
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab196675 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Anoctamin 7 also known as ANO7 or TMEM16G is a transmembrane protein that belongs to the anoctamin family. It functions as a calcium-activated chloride channel with a role in ion transport. Anoctamin 7 has a molecular weight of approximately 120 kDa. The protein is expressed in many tissues with high levels in the prostate suggesting a specialized function in this organ.
- Biological function summary
ANO7 influences important cellular processes like fluid secretion cell volume regulation and signal transduction. It is a component of a larger protein complex involved in calcium signaling which impacts various physiological functions. In the prostate ANO7 expression may influence normal prostate function and development indicating a possible role in prostate-specific tasks.
- Pathways
ANO7 participates in important pathways like calcium-dependent regulation of ion transport and signal transduction through chloride channels. It interacts with proteins such as ANO1 and ANO2 within these pathways sharing similar chloride channel functions. These interactions are necessary for maintaining ion balance and signaling across cell membranes impacting general cellular homeostasis.
- Associated diseases and disorders
ANO7's expression has been linked to prostate cancer where abnormalities in its function may influence disease development or progression. The protein is also associated with disorders related to ion transport potentially due to its role in chloride channel regulation. Research on ANO7 interactions with other proteins like ANO1 might reveal further insights into its contribution to these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Anoctamin 7 antibody - C-terminal (ab196675)
All lanes: Western blot - Anti-Anoctamin 7 antibody - C-terminal (ab196675) at 1/500 dilution
Lane 1: LOVO cell extract
Lane 2: LOVO cell extract with immunizing peptide
Predicted band size: 106 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anoctamin 7 antibody - C-terminal (ab196675)
Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling Anoctamin 7 with ab196675 at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
Immunocytochemistry/ Immunofluorescence - Anti-Anoctamin 7 antibody - C-terminal (ab196675)
Immunofluorescent analysis of HepG2 cells labeling Anoctamin 7 with ab196675 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
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