AB250456

Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ANP32C/PP32R1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PP32R1, ANP32CP, Putative uncharacterized protein ANP32CP, Acidic leucine-rich nuclear phosphoprotein 32 family member C, Phosphoprotein 32-related protein 1, Tumorigenic protein pp32r1

4 Images

Flow Cytometry (Intracellular) - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

This data was developed using ab181242, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C/PP32R1 with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

This data was developed using ab181242, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C/PP32R1 with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Supplier Data

Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

This data was developed using ab181242, the same antibody clone in a different buffer formulation.

Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] (<a href='/en-us/products/primary-antibodies/anp32c-pp32r1-antibody-epr13354-ab181242'>ab181242</a>)

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Supplier Data

Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

This data was developed using ab181242, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] (<a href='/en-us/products/primary-antibodies/anp32c-pp32r1-antibody-epr13354-ab181242'>ab181242</a>) at 1/10000 dilution

Lane 1:

HUVEC cell lysate at 20 µg

Lane 2:

PC-3 cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Unconjugated

Anti-ANP32C/PP32R1 antibody [EPR13354]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13354

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250456 is the carrier-free version of ab181242.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANP32C also known as PP32R1 is a protein with a mass of approximately 30 kDa. It belongs to the acidic nuclear phosphoprotein 32 kDa family and plays mechanical roles in several cellular processes. ANP32C localizes mainly in the nucleus and shows expression in various tissues with notable presence in the brain and peripheral blood. Its functions involve interactions with other proteins to regulate cellular processes making it a significant target for research in cellular biology.

Biological function summary

ANP32C influences multiple cellular events including regulation of gene expression apoptosis and chromatin modification. It is part of several multiprotein complexes which contribute to its role in transcriptional regulation and cell cycle progression. The interaction of ANP32C with histone acetyltransferases plays a significant role in chromatin remodeling affecting gene expression patterns. This protein's actions are critical for maintaining normal cellular functions and responding to stress conditions.

Pathways

ANP32C engages in the apoptotic and inflammatory signaling pathways. It associates with proteins such as caspases and NF-kappa B influencing cell survival and immune responses. ANP32C's participation in the apoptotic pathway reflects its role in regulating programmed cell death a fundamental process for tissue homeostasis and development. Its interaction with inflammatory mediators highlights its contribution to immune responses and potential implications in inflammatory conditions.

ANP32C has connections to cancer and neurodegenerative diseases. Abnormal expression or mutation of ANP32C correlates with increased proliferation in some cancers emphasizing its role in tumorigenesis. Additionally the protein links with tau pathology implicating it in neurodegenerative disorders such as Alzheimer's disease. These associations suggest that ANP32C along with interacting proteins like tau may serve as potential therapeutic targets for disease intervention strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information ANP32CP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

Immunocytochemistry/ Immunofluorescence - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (AB250456)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise