Anti-Anterior Gradient 2 antibody [EPR3278] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] - BSA and Azide free (AB181829)

ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76473).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Anterior Gradient 2 antibody [EPR3278] - BSA and Azide free (AB181829)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Anterior Gradient 2 with purified ab76473 at 1/100 dilution (3.5 μg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76473).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] - BSA and Azide free (AB181829)

ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76473).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Anterior Gradient 2 antibody [EPR3278] - BSA and Azide free (AB181829)

This data was developed using ab76473, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Anterior Gradient 2 with purified ab76473 at 1/40 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

• IP

Lab

Immunoprecipitation - Anti-Anterior Gradient 2 antibody [EPR3278] - BSA and Azide free (AB181829)

This data was developed using ab76473, the same antibody clone in a different buffer formulation.
Purified ab76473 at 1/20 dilution (2μg) immunoprecipitating Anterior Gradient 2 in HT-29 whole cell lysate.
Lane 1 (input) : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab76473 + HT-29 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76473 in HT-29 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 17 kDa

All lanes:

Immunoprecipitation - Anti-Anterior Gradient 2 antibody [EPR3278] (<a href='/en-us/products/primary-antibodies/anterior-gradient-2-antibody-epr3278-ab76473'>ab76473</a>)

Predicted band size: 19 kDa

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