AB230981

Anti-Antithrombin III/ATIII antibody

Name: Anti-Antithrombin III/ATIII antibody (ab230981) | Abcam
Brand: Abcam Limited
SKU: AB230981
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Antithrombin III/ATIII antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Rat, Rat, Human samples. Cited in 1 publication.

View Alternative Names

AT3, PRO0309, SERPINC1, Antithrombin-III, ATIII, Serpin C1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody (AB230981)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody (AB230981)

Formalin-fixed, paraffin-embedded rat liver tissue stained for Antithrombin III/ATIII with ab230981 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

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Western blot - Anti-Antithrombin III/ATIII antibody (ab230981) at 1 µg/mL

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Human serum

Predicted band size: 35 kDa,39 kDa,40 kDa,44 kDa,47 kDa,50 kDa,52 kDa,60 kDa,69 kDa

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Supplier Data

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

All lanes:

Western blot - Anti-Antithrombin III/ATIII antibody (ab230981) at 1 µg/mL

All lanes:

Rat serum

Predicted band size: 44 kDa,52 kDa

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Supplier Data

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

All lanes:

Western blot - Anti-Antithrombin III/ATIII antibody (ab230981) at 1 µg/mL

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Rat lung lysate

Predicted band size: 52 kDa

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Supplier Data

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

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Western blot - Anti-Antithrombin III/ATIII antibody (ab230981) at 2 µg/mL

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Recombinant rat Antithrombin III/ATIII

Predicted band size: 52 kDa

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Supplier Data

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

All lanes:

Western blot - Anti-Antithrombin III/ATIII antibody (ab230981) at 2 µg/mL

All lanes:

Rat cerebrum lysate

Predicted band size: 38 kDa,39 kDa,44 kDa,45 kDa,48 kDa,49 kDa,52 kDa,70 kDa,81 kDa

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Supplier Data

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

All lanes:

Western blot - Anti-Antithrombin III/ATIII antibody (ab230981) at 1 µg/mL

All lanes:

Rat liver lysate

Predicted band size: 52 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Recombinant fragment - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Antithrombin III also known as ATIII or antithrombin 3 is a glycoprotein with a mass of approximately 58 kDa. This protein is mainly synthesized in the liver and circulates in the blood plasma. Antithrombin III functions as a serine protease inhibitor targeting and inhibiting several key enzymes involved in blood coagulation including thrombin and Factor Xa. Its activity is influenced by the presence of heparin which increases its inhibitory effects on these enzymes. This interaction is essential for regulating blood clot formation and maintaining hemostatic balance.

Biological function summary

Antithrombin III plays a critical role in blood clotting by specifically inhibiting clotting factors. It forms complexes with its target enzymes leading to their inactivation and preventing excessive thrombin production. The protein does not form part of larger complexes but acts independently to exert its anticoagulant function. The normal range for antithrombin 3 levels in plasma is important for ensuring a balanced blood coagulation system. Its function helps prevent inappropriate clot formation and ensures smooth blood flow through the circulatory system.

Pathways

Antithrombin III is integral to the coagulation cascade. It is primarily involved in the intrinsic and common coagulation pathways where it regulates thrombin and Factor Xa activity. This regulation prevents the unchecked formation of fibrin clots which can lead to thrombosis. ATIII works closely with proteins like heparin cofactor II and protein C which also modulate the coagulation pathway emphasizing its role in maintaining hemostatic balance. Its function is essential in controlling the coagulation cascade and ensuring that it proceeds appropriately without leading to pathology.

Antithrombin III deficiency can lead to coagulation disorders such as thrombophilia characterized by an increased risk of forming clots. Inherited or acquired deficiencies in antithrombin 3 can result in life-threatening thrombotic events. Furthermore liver diseases which affect the production of ATIII can also result in decreased antithrombin activity compounding coagulation problems. Other proteins related to this process include protein S and plasminogen which also play roles in clot regulation and fibrinolysis. Understanding the connection of antithrombin III to these pathways and diseases is important for developing effective therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade (PubMed : 15140129, PubMed : 15853774). AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa (PubMed : 15140129). Its inhibitory activity is greatly enhanced in the presence of heparin.

See full target information SERPINC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1324205 PubMed38090559

2023

Lipid metabolism-related gene expression in the immune microenvironment predicts prognostic outcomes in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Bingbiao Lin,Huikun Chen,Yinyan Ye,Yijie Huang,Zhen Chen,Jun Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Antithrombin III/ATIII antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody (AB230981)

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

Western blot - Anti-Antithrombin III/ATIII antibody (AB230981)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise