Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)

Rabbit Recombinant Monoclonal Antithrombin III/ATIII antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (AB314549), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Expected	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Tested	Expected	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Serpinc1

Function

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin (By similarity).

Alternative names

At3, Serpinc1, Antithrombin-III, ATIII, Serpin C1

Recommended products

Rabbit Recombinant Monoclonal Antithrombin III/ATIII antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28171-25
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Antithrombin III also known as ATIII or antithrombin 3 is a glycoprotein with a mass of approximately 58 kDa. This protein is mainly synthesized in the liver and circulates in the blood plasma. Antithrombin III functions as a serine protease inhibitor targeting and inhibiting several key enzymes involved in blood coagulation including thrombin and Factor Xa. Its activity is influenced by the presence of heparin which increases its inhibitory effects on these enzymes. This interaction is essential for regulating blood clot formation and maintaining hemostatic balance.

Biological function summary

Antithrombin III plays a critical role in blood clotting by specifically inhibiting clotting factors. It forms complexes with its target enzymes leading to their inactivation and preventing excessive thrombin production. The protein does not form part of larger complexes but acts independently to exert its anticoagulant function. The normal range for antithrombin 3 levels in plasma is important for ensuring a balanced blood coagulation system. Its function helps prevent inappropriate clot formation and ensures smooth blood flow through the circulatory system.

Pathways

Antithrombin III is integral to the coagulation cascade. It is primarily involved in the intrinsic and common coagulation pathways where it regulates thrombin and Factor Xa activity. This regulation prevents the unchecked formation of fibrin clots which can lead to thrombosis. ATIII works closely with proteins like heparin cofactor II and protein C which also modulate the coagulation pathway emphasizing its role in maintaining hemostatic balance. Its function is essential in controlling the coagulation cascade and ensuring that it proceeds appropriately without leading to pathology.

Associated diseases and disorders

Antithrombin III deficiency can lead to coagulation disorders such as thrombophilia characterized by an increased risk of forming clots. Inherited or acquired deficiencies in antithrombin 3 can result in life-threatening thrombotic events. Furthermore liver diseases which affect the production of ATIII can also result in decreased antithrombin activity compounding coagulation problems. Other proteins related to this process include protein S and plasminogen which also play roles in clot regulation and fibrinolysis. Understanding the connection of antithrombin III to these pathways and diseases is important for developing effective therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: brain.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 3609301, PMID: 25485983, PMID: 35935621).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 26 seconds, lanes 3-4: 81 seconds, lanes 5-6: 180 seconds
All lanes: Western blot - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat liver tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Human liver tissue lysate at 20 µg
Lane 6: Human cerebellum tissue lysate at 20 µg
Secondary
Lanes 1 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lanes 5 - 6: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 51 kDa, 52 kDa, 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Antithrombin III/ATIII with ab314549 at 1/2000 (0.263 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: No staining on human cerebrum. The section was incubated with ab314549 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Antithrombin III/ATIII with ab314549 at 1/2000 (0.263 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: No staining on rat cerebrum. The section was incubated with ab314549 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Antithrombin III/ATIII with ab314549 at 1/2000 (0.263 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue: Weak staining in plasma of mouse cerebrum. The section was incubated with ab314549 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Antithrombin III/ATIII with ab314549 at 1/2000 (0.263 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on plasma of rat kidney. The section was incubated with ab314549 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Antithrombin III/ATIII with ab314549 at 1/2000 (0.263 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on plasma of mouse kidney. The section was incubated with ab314549 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Antithrombin III/ATIII with ab314549 at 1/2000 (0.263 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on plasma of human kidney. The section was incubated with ab314549 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunoprecipitation - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Antithrombin III/ATIII was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab314549 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314549 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: ab314549 IP in Mouse liver tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314549 in mouse liver tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549) at 1/30 dilution
All lanes: Mouse liver tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 76s
Western blot - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 3609301, PMID: 25485983, PMID: 35935621).
All lanes: Western blot - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549) at 1/1000 dilution
All lanes: Human plasma lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 51 kDa, 52 kDa
Exposure time: 180s
Western blot - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 30237862, PMID: 360930, PMID: 25485983, PMID: 35935621).
All lanes: Western blot - Anti-Antithrombin III/ATIII antibody [EPR28171-25] (ab314549) at 1/1000 dilution
All lanes: Mouse serum lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 51 kDa, 52 kDa, 90 kDa
Exposure time: 3s