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AB231342

Anti-Antizyme inhibitor 1 antibody

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(1 Publication)

Rabbit Polyclonal Antizyme inhibitor 1 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AZIN1 aa 1-300.

View Alternative Names

OAZI, OAZIN, AZIN1, Antizyme inhibitor 1, AZI, AZI1, Ornithine decarboxylase antizyme inhibitor

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antizyme inhibitor 1 antibody (AB231342)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antizyme inhibitor 1 antibody (AB231342)

Paraffin-embedded human stomach tissue stained for Antizyme inhibitor 1 using ab231342 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antizyme inhibitor 1 antibody (AB231342)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antizyme inhibitor 1 antibody (AB231342)

Paraffin-embedded human liver tissue stained for Antizyme inhibitor 1 using ab231342 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Antizyme inhibitor 1 antibody (AB231342)
  • WB

Supplier Data

Western blot - Anti-Antizyme inhibitor 1 antibody (AB231342)

All lanes:

Western blot - Anti-Antizyme inhibitor 1 antibody (ab231342) at 1 µg/mL

Lane 1:

Pig liver lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 50 kDa

false

Western blot - Anti-Antizyme inhibitor 1 antibody (AB231342)
  • WB

Supplier Data

Western blot - Anti-Antizyme inhibitor 1 antibody (AB231342)

All lanes:

Western blot - Anti-Antizyme inhibitor 1 antibody (ab231342) at 1 µg/mL

All lanes:

Recombinant human Antizyme inhibitor 1 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human AZIN1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O14977

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Complementary Products

Primary Antibodies

AB223481

Anti-OAZ1 antibody

Western blot - Anti-OAZ1 antibody (AB223481)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB162598

Recombinant Human Antizyme inhibitor 1 protein

SDS-PAGE - Recombinant Human Antizyme inhibitor 1 protein (AB162598)

  • 0
  • 0
Proteins & Peptides

AB156968

Recombinant Human KRAS protein

SDS-PAGE - Recombinant Human KRAS protein (AB156968)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231342 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Antizyme inhibitor 1 (AZIN1) also known as ornithine decarboxylase antizyme inhibitor (OAZI) is a regulatory protein that modulates polyamine levels in the cell. The mass of AZIN1 is approximately 50 kDa. It is a cytoplasmic enzyme and expressed in various tissues including the liver and pancreas. AZIN1 competes with ornithine decarboxylase (ODC) for binding to antizyme which is a negative regulator of ODC. Unlike ODC AZIN1 lacks catalytic activity but plays an important role in cellular regulation.
Biological function summary

AZIN1 significantly influences polyamine biosynthesis by preventing the formation of inhibitory antizyme-ODC complexes. This effectively regulates polyamine levels that are important for cell growth and proliferation. AZIN1 also interacts with other proteins such as ODC forming a regulatory system to balance polyamine homeostasis. The dynamic interaction within this system is critical for cell function affecting several metabolic processes and cellular activities.

Pathways

AZIN1 integrates importantly into the polyamine biosynthesis pathway. This pathway is essential for providing necessary polyamines like putrescine spermidine and spermine central for cellular replication and differentiation. AZIN1's activity can indirectly influence related signaling pathways through polyamine availability impacting proteins like c-Myc which is known to play roles in cell cycle regulation. The control of polyamine metabolism by AZIN1 reflects its broader role in cellular signaling networks.

AZIN1 exhibits associations with cancer and liver fibrosis. Abnormal expression or mutations in AZIN1 impact liver disease progression by mediating fibrosis-associated pathways. Furthermore AZIN1's involvement in regulating polyamine levels has links to oncogenesis with overexpressed AZIN1 promoting tumor development and progression. AZIN1's interaction with antizyme and the disruption of this interaction can contribute to pathological conditions by impairing normal cellular regulatory mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding (PubMed : 17900240, PubMed : 26305948). Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production (PubMed : 17900240).
See full target information AZIN1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:934819 PubMed36148236

2022

induces autophagy in bovine mammary epithelial cells the HIF-1α and AMPKα/ULK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenpeng Zhao,Maolin Xu,Herman W Barkema,Xiaochen Xie,Yushan Lin,Sohrab Khan,John P Kastelic,Dong Wang,Zhaoju Deng,Bo Han
View all publications
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Product promise

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