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AB189995

Anti-AP-2 complex subunit alpha-1 antibody

5

(1 Review)

|

(9 Publications)

Goat Polyclonal AP-2 complex subunit alpha-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human AP2A1 aa 700 to C-terminus.

View Alternative Names

ADTAA, CLAPA1, AP2A1, AP-2 complex subunit alpha-1, 100 kDa coated vesicle protein A, Adaptor protein complex AP-2 subunit alpha-1, Adaptor-related protein complex 2 subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin assembly protein complex 2 alpha-A large chain, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling AP-2 complex subunit alpha-1 with ab189995 at 5μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)

Immunofluorescent analysis of methanol-fixed HeLa cells stably expressing a chimeric construct including the brain-specific splice variant of Mouse AP-2 complex subunit alpha-1 labeling AP-2 complex subunit alpha-1 with ab189995 at 0.1 μg/ml.

Western blot - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)
  • WB

Supplier Data

Western blot - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)

The left panel is stained with Ponceau red.

Lanes 1 - 3:

total protein blot stained with Ponceau Red

Lanes 4 - 6:

Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab189995) at 0.1 µg/mL

Lanes 1 and 4:

Mouse Liver lysate at 5 µg

Lanes 2 and 5:

Mouse Brain lysate at 5 µg

Lanes 3 and 6:

Mouse Kidney lysate at 5 µg

Predicted band size: 108 kDa

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Western blot - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)
  • WB

Supplier Data

Western blot - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)

All lanes:

Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab189995) at 0.01 µg/mL

All lanes:

Human Frontal Cortex lysate at 35 µg

Predicted band size: 108 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human AP2A1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95782

Specificity

ab189995 is expected to recognize reported isoform 1 (NP_055018.2) only.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AP-2 complex subunit alpha-1 also known as AP2A1 is a protein subunit part of the adaptor protein complex 2 (AP2) involved in clathrin-mediated endocytosis. The protein has a mass of about 105 kDa and is expressed in various tissues including the brain and lung. It mediates interactions between cargo proteins and clathrin facilitating the formation of clathrin-coated vesicles. By binding to phosphoinositides and specific motifs in the cargo proteins it regulates their sequestration into vesicles making it a central player in cellular trafficking.
Biological function summary

AP2A1 plays a critical role in vesicle-mediated transport by forming part of the AP2 complex which includes other subunits such as beta μ and sigma subunits. This complex ensures the specific and efficient endocytosis of receptor proteins and lipids influencing receptor recycling and signaling. Its participation in the AP2 complex affects cellular functions like nutrient uptake signal transduction and membrane protein turnover highlighting its functional diversity within cellular processes.

Pathways

The function of AP2A1 integrates into the clathrin-mediated endocytic pathway and the receptor-mediated endocytosis pathway. These pathways are essential for internalizing nutrients hormones and other signaling molecules. The AP2 complex collaborates with proteins such as clathrin and dynamin facilitating clathrin-coated vesicle formation and subsequent vesicle scission from the plasma membrane. The association with dynamin highlights AP2A1's role in vesicle budding and release reflecting its significance in membrane trafficking and cellular homeostasis.

AP2A1 has connections to neurological disorders and cancer. Disruptions in the clathrin-mediated endocytic functions attributed to AP2A1 anomalies can lead to neurological diseases like Alzheimer's disease due to impaired amyloid-beta precursor protein processing. Abnormal endocytic trafficking can also influence cancer progression as receptor signaling variances affect cell proliferation and survival. The interaction of AP2A1 within these pathways involves proteins such as amyloid precursor protein in Alzheimer's and various receptor tyrosine kinases in cancer underlining its pathological relevance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed : 23676497). The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).
See full target information AP2A1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1250920 PubMed38077388

2023

Autophagy-mediated NKG2D internalization impairs NK cell function and exacerbates radiation pneumonitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqing Wang,Xinyue Ma,Xinyu Zhang,Dizhi Jiang,Hongyuan Mao,Zerun Li,Yu Tian,Bo Cheng

Nature 609:128-135 PubMed35978188

2022

Endocytosis in the axon initial segment maintains neuronal polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsie Eichel,Takeshi Uenaka,Vivek Belapurkar,Rui Lu,Shouqiang Cheng,Joseph S Pak,Caitlin A Taylor,Thomas C Südhof,Robert Malenka,Marius Wernig,Engin Özkan,David Perrais,Kang Shen

ACS nano 16:7559-7571 PubMed35533401

2022

Expansion Microscopy for Imaging the Cell-Material Interface.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa L Nakamoto,Csaba Forró,Wei Zhang,Ching-Ting Tsai,Bianxiao Cui

Nature communications 12:4502 PubMed34301937

2021

YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Feliciano,Carolyn M Ott,Isabel Espinosa-Medina,Aubrey V Weigel,Lorena Benedetti,Kristin M Milano,Zhonghua Tang,Tzumin Lee,Harvey J Kliman,Seth M Guller,Jennifer Lippincott-Schwartz

Frontiers in cardiovascular medicine 8:678401 PubMed34239903

2021

Elastogenesis Correlates With Pigment Production in Murine Aortic Valve Leaflets.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua D Hutcheson,Florian Schlotter,Michael D Creager,Xiaoshuang Li,Tan Pham,Payal Vyas,Hideyuki Higashi,Simon C Body,Masanori Aikawa,Sasha A Singh,Lidia Kos,Elena Aikawa

Molecular biology of the cell 31:2475-2493 PubMed32816642

2020

Novel function for AP-1B during cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Margaret Johnson Kell,Su Fen Ang,Lucy Pigati,Abby Halpern,Heike Fölsch

Experimental cell research 385:111647 PubMed31562859

2019

BMP2-mediated PTEN enhancement promotes differentiation of hair follicle stem cells by inducing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Bingjie Cai,Yunpeng Zheng,Jiadi Yan,Junmin Wang,Xiaojun Liu,Guangwen Yin

PLoS genetics 14:e1007491 PubMed29975682

2018

mTOR acts as a pivotal signaling hub for neural crest cells during craniofacial development.

Applications

Unspecified application

Species

Unspecified reactive species

Xuguang Nie,Jinxuan Zheng,Christopher L Ricupero,Ling He,Kai Jiao,Jeremy J Mao

Nature communications 8:14612 PubMed28266544

2017

Misrouting of v-ATPase subunit V0a1 dysregulates lysosomal acidification in a neurodegenerative lysosomal storage disease model.

Applications

ICC/IF, ICC/IF, IP, IP

Species

Mouse, Human, Mouse, Human

Maria B Bagh,Shiyong Peng,Goutam Chandra,Zhongjian Zhang,Satya P Singh,Nagarajan Pattabiraman,Aiyi Liu,Anil B Mukherjee
View all publications
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