AB170955

Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal AP-2 complex subunit alpha-1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ADTAA, CLAPA1, AP2A1, AP-2 complex subunit alpha-1, 100 kDa coated vesicle protein A, Adaptor protein complex AP-2 subunit alpha-1, Adaptor-related protein complex 2 subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin assembly protein complex 2 alpha-A large chain, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

1 Images

Supplier Data

Western blot - Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] (AB170955)

All lanes:

Western blot - Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] (ab170955) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 108 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7573(2)(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AP-2 complex subunit alpha-1 also known as AP2A1 is a protein subunit part of the adaptor protein complex 2 (AP2) involved in clathrin-mediated endocytosis. The protein has a mass of about 105 kDa and is expressed in various tissues including the brain and lung. It mediates interactions between cargo proteins and clathrin facilitating the formation of clathrin-coated vesicles. By binding to phosphoinositides and specific motifs in the cargo proteins it regulates their sequestration into vesicles making it a central player in cellular trafficking.

Biological function summary

AP2A1 plays a critical role in vesicle-mediated transport by forming part of the AP2 complex which includes other subunits such as beta μ and sigma subunits. This complex ensures the specific and efficient endocytosis of receptor proteins and lipids influencing receptor recycling and signaling. Its participation in the AP2 complex affects cellular functions like nutrient uptake signal transduction and membrane protein turnover highlighting its functional diversity within cellular processes.

Pathways

The function of AP2A1 integrates into the clathrin-mediated endocytic pathway and the receptor-mediated endocytosis pathway. These pathways are essential for internalizing nutrients hormones and other signaling molecules. The AP2 complex collaborates with proteins such as clathrin and dynamin facilitating clathrin-coated vesicle formation and subsequent vesicle scission from the plasma membrane. The association with dynamin highlights AP2A1's role in vesicle budding and release reflecting its significance in membrane trafficking and cellular homeostasis.

AP2A1 has connections to neurological disorders and cancer. Disruptions in the clathrin-mediated endocytic functions attributed to AP2A1 anomalies can lead to neurological diseases like Alzheimer's disease due to impaired amyloid-beta precursor protein processing. Abnormal endocytic trafficking can also influence cancer progression as receptor signaling variances affect cell proliferation and survival. The interaction of AP2A1 within these pathways involves proteins such as amyloid precursor protein in Alzheimer's and various receptor tyrosine kinases in cancer underlining its pathological relevance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed : 23676497). The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).

See full target information AP2A1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular immunology 357:104203 PubMed32977155

2020

Full-length IL-33 regulates Smad3 phosphorylation and gene transcription in a distinctive AP2-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Irina G Luzina,Rita Fishelevich,Brian S Hampton,Jean-Paul Courneya,Francesca R Parisella,Katerina N Lugkey,Frank X Baleno,Dohyun Choi,Pavel Kopach,Virginia Lockatell,Nevins W Todd,Sergei P Atamas

Nucleic acids research 48:6672-6684 PubMed32504494

2020

Pathological mutations in PNKP trigger defects in DNA single-strand break repair but not DNA double-strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Ilona Kalasova,Richard Hailstone,Janin Bublitz,Jovel Bogantes,Winfried Hofmann,Alejandro Leal,Hana Hanzlikova,Keith W Caldecott

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)]

Western blot - Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] (AB170955)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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