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Rabbit Recombinant Monoclonal AP-2 complex subunit alpha-1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] - BSA and Azide free (AB249620), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Predicted
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Predicted
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).

Alternative names

ADTAA, CLAPA1, AP2A1, AP-2 complex subunit alpha-1, 100 kDa coated vesicle protein A, Adaptor protein complex AP-2 subunit alpha-1, Adaptor-related protein complex 2 subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin assembly protein complex 2 alpha-A large chain, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

Recommended products

Rabbit Recombinant Monoclonal AP-2 complex subunit alpha-1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7573(2)(B)
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249620 is the carrier-free version of Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] ab170955.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The AP-2 complex subunit alpha-1 also known as AP2A1 is a protein subunit part of the adaptor protein complex 2 (AP2) involved in clathrin-mediated endocytosis. The protein has a mass of about 105 kDa and is expressed in various tissues including the brain and lung. It mediates interactions between cargo proteins and clathrin facilitating the formation of clathrin-coated vesicles. By binding to phosphoinositides and specific motifs in the cargo proteins it regulates their sequestration into vesicles making it a central player in cellular trafficking.

Biological function summary

AP2A1 plays a critical role in vesicle-mediated transport by forming part of the AP2 complex which includes other subunits such as beta μ and sigma subunits. This complex ensures the specific and efficient endocytosis of receptor proteins and lipids influencing receptor recycling and signaling. Its participation in the AP2 complex affects cellular functions like nutrient uptake signal transduction and membrane protein turnover highlighting its functional diversity within cellular processes.

Pathways

The function of AP2A1 integrates into the clathrin-mediated endocytic pathway and the receptor-mediated endocytosis pathway. These pathways are essential for internalizing nutrients hormones and other signaling molecules. The AP2 complex collaborates with proteins such as clathrin and dynamin facilitating clathrin-coated vesicle formation and subsequent vesicle scission from the plasma membrane. The association with dynamin highlights AP2A1's role in vesicle budding and release reflecting its significance in membrane trafficking and cellular homeostasis.

Associated diseases and disorders

AP2A1 has connections to neurological disorders and cancer. Disruptions in the clathrin-mediated endocytic functions attributed to AP2A1 anomalies can lead to neurological diseases like Alzheimer's disease due to impaired amyloid-beta precursor protein processing. Abnormal endocytic trafficking can also influence cancer progression as receptor signaling variances affect cell proliferation and survival. The interaction of AP2A1 within these pathways involves proteins such as amyloid precursor protein in Alzheimer's and various receptor tyrosine kinases in cancer underlining its pathological relevance in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] - BSA and Azide free (ab249620), expandable thumbnail

    Western blot - Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] - BSA and Azide free (ab249620)

    This data was developed using Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] ab170955, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] (Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] ab170955) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: A431 cell lysate at 10 µg

    Lane 3: 293T cell lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 108 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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