AB139179

Anti-AP-A antibody [EPR9410]

Name: Anti-AP-A antibody [EPR9410] (ab139179) | Abcam
Brand: Abcam Limited
SKU: AB139179
Price: 365 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal AP-A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD249, Glutamyl aminopeptidase, EAP, Aminopeptidase A, Differentiation antigen gp160, AP-A, ENPEP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9410] (AB139179)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9410] (AB139179)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling AP-A with ab139179 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Western blot - Anti-AP-A antibody [EPR9410] (AB139179)

All lanes:

Western blot - Anti-AP-A antibody [EPR9410] (ab139179) at 1/1000 dilution

Lane 1:

Human fetal lung tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 109 kDa

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Western blot - Anti-AP-A antibody [EPR9410] (AB139179)

All lanes:

Western blot - Anti-AP-A antibody [EPR9410] (ab139179) at 1/1000 dilution

All lanes:

Human small intestine lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 109 kDa

false

Carrier free

Anti-AP-A antibody [EPR9410] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9410

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP-A also known as Aminopeptidase A is an enzyme that plays a critical role in cellular processes by cleaving N-terminal amino acids from peptide substrates. It has a molecular mass of approximately 100 kDa. AP-A is highly expressed in the brain particularly in the hippocampus and glial cells as well as in other tissues like the kidney and placenta. Its activity has been observed in the synaptic membranes signifying its importance in neurobiological functions.

Biological function summary

Aminopeptidase A participates in the regulation of neuropeptides and peptide hormones. It forms part of the renin-angiotensin system where it influences blood pressure and fluid balance. By converting angiotensin II to angiotensin III AP-A modulates these physiological processes. Moreover it is involved in degrading enkephalins which impacts pain perception and emotional responses. This enzyme interacts with other aminopeptidases in the body contributing to various cellular functions.

Pathways

AP-A significantly impacts the renin-angiotensin system and the endothelin pathway. It influences the cardiovascular system by processing angiotensin peptides working closely with proteins such as angiotensin-converting enzyme (ACE). In the endothelin pathway AP-A affects peptide regulation and is associated with the activity of endothelin-converting enzyme. These pathways demonstrate the enzyme's role in cardiovascular health and homeostasis.

Aminopeptidase A is linked to hypertension and neurological disorders like Alzheimer's disease. Increased activity or expression of AP-A is often seen in the context of high blood pressure where it works in conjunction with ACE to modulate blood pressure levels. In Alzheimer's disease altered AP-A activity affects neuropeptide regulation linking it to beta-amyloid accumulation. These associations highlight the enzyme's potential as a target for therapeutic interventions in hypertension and neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.

See full target information ENPEP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cardiology research and practice 2023:8141129 PubMed36741296

2023

Recombinant Adenovirus siRNA Knocking Down the Ndufs4 Gene Alleviates Myocardial Apoptosis Induced by Oxidative Stress Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Wang,Jinsheng Zhang,Aijun Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AP-A antibody [EPR9410]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [EPR9410] (AB139179)

Western blot - Anti-AP-A antibody [EPR9410] (AB139179)

Western blot - Anti-AP-A antibody [EPR9410] (AB139179)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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