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AB326687

Anti-AP-A antibody [SR1222]

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Rabbit Recombinant Monoclonal AP-A antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

CD249, Glutamyl aminopeptidase, EAP, Aminopeptidase A, Differentiation antigen gp160, AP-A, ENPEP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [SR1222] (AB326687)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody [SR1222] (AB326687)

Formalin-fixed, paraffin-embedded human kidney tissue stained for AAP-A using antibody at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-AP-A antibody [SR1222] (AB326687)
  • WB

Supplier Data

Western blot - Anti-AP-A antibody [SR1222] (AB326687)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 109 kDa
Observed band size : 160 kDa

Exposure time : 4 seconds

All lanes:

Western blot - Anti-AP-A antibody [SR1222] (ab326687) at 1/1000 dilution

All lanes:

Human fetal lung lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 109 kDa

Observed band size: 160 kDa

false

Exposure time: 4s

Western blot - Anti-AP-A antibody [SR1222] (AB326687)
  • WB

Supplier Data

Western blot - Anti-AP-A antibody [SR1222] (AB326687)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : Aminopeptidase A / CD249 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-AP-A antibody [SR1222] (ab326687) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

Aminopeptidase A / CD249 knockdown Hela cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1222

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
See full target information ENPEP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com