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AB241289

Anti-AP-B antibody

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(1 Publication)

Rabbit Polyclonal AP-B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RNPEP aa 600 to C-terminus.

View Alternative Names

APB, RNPEP, Aminopeptidase B, AP-B, Arginine aminopeptidase, Arginyl aminopeptidase

2 Images
Immunoprecipitation - Anti-AP-B antibody (AB241289)
  • IP

Supplier Data

Immunoprecipitation - Anti-AP-B antibody (AB241289)

AP-B was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241289 at 8 μl/reaction. Western blot was performed from the immunoprecipitate using ab241289 at 1/1000 dilution.

Lane 1 : ab241289 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-AP-B antibody (ab241289)

Predicted band size: 73 kDa

false

Western blot - Anti-AP-B antibody (AB241289)
  • WB

Supplier Data

Western blot - Anti-AP-B antibody (AB241289)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-AP-B antibody (ab241289) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 73 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RNPEP aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H4A4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 8 - 15 μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241289 was affinity purified using an epitope specific to AP-B immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AP-B also known as Alkaline Phosphatase B plays a critical role in dephosphorylation processes. This protein weighs approximately 68 kDa. Researchers find AP-B expressed in various tissues including liver bone kidneys and the placenta. The enzyme functions optimally in an alkaline environment and serves different roles depending on its location. AP-B's activity in these tissues highlights its diverse functional capacity from bone mineralization to liver metabolism.
Biological function summary

The role of this enzyme impacts the regulation of phosphate groups on peptides and other molecules. AP-B often forms part of larger enzymatic complexes contributing to the deactivation of molecules by removing phosphate groups. This action influences cell growth differentiation and signal transduction. Additionally due to its expression in the intestines AP-B also plays a role in digestion aiding in nutrient absorption by catalyzing the breakdown of nucleotides.

Pathways

Several lines of evidence show that AP-B integrates into the bone Gla protein pathway and the hepatic detoxification pathway. In bone Gla protein synthesis AP-B interacts with osteocalcin key for bone mineralization and calcium binding. Within the detoxification pathway in hepatic systems AP-B aids in processing various xenobiotics and endogenous substrates. Coordination with these proteins underlines AP-B's role in maintaining homeostasis and its enzymatic importance in metabolic pathways.

AP-B levels correlate with conditions such as Paget's disease of bone and hyperparathyroidism. For instance elevated AP-B activity is often a marker for increased osteoblastic activity in Paget's disease where malfunction leads to excessive bone resorption and formation. Moreover hyperparathyroidism can result in augmented activity of AP-B linked to disturbances in calcium and phosphate balance. In these conditions AP-B interacts with proteins such as parathyroid hormone which influence bone remodeling and calcium homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4) (By similarity).
See full target information RNPEP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e39859 PubMed39553628

2024

Comparative proteomic analysis between tumor tissues and intratumoral exosomes from lung adenocarcinoma patients identifies PAFAH1B3 as an exosomal protein key for initiating metastasis in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Wang,Ling Xiao,Ling Gao,Jia Wu,Siliang Wang,Miao-Miao Zheng,Chen-Tai Qin,Xian-Ge Huang,Lei Zhou,Wei-Jie Xu,He-Gen Li,Wen-Lian Chen,Li-Hua Zhu,Xing Jin
View all publications
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Product promise

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