Skip to main content

Rabbit Recombinant Monoclonal POLD1 antibody - conjugated to Alkaline Phosphatase.

Be the first to review this product! Submit a review

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alkaline Phosphatase

Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.016% Magnesium chloride, 0.001% Zinc chloride

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites (PubMed:19074196, PubMed:24191025).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal POLD1 antibody - conjugated to Alkaline Phosphatase.

Alternative names

Key facts

Isotype

IgG

Conjugation

Alkaline Phosphatase

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR15118

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

POLD1 also known as DNA polymerase delta is a catalytic subunit of the DNA polymerase delta complex with alternative names such as delta 647 delta 555 delta 488 delta APC delta ALP and ADN-594. Its mass is approximately 124 kDa. POLD1 is expressed in various tissues but shows high expression in proliferating cells. The primary role is to facilitate the high-fidelity DNA replication process by synthesizing the lagging strand during DNA replication and repair. Its enzymatic activity involves adding nucleotides to a primer strand of DNA and executing proofreading functions to ensure replication accuracy.

Biological function summary

POLD1 is integral to the replication machinery where it functions as part of the multi-subunit DNA polymerase delta complex. This complex plays a pivotal role in promoting genome stability by participating in DNA synthesis proofreading and repair processes. The complex provides both polymerase and exonuclease activity ensuring accuracy during DNA replication. Besides POLD1 interacts with PCNA (proliferating cell nuclear antigen) to enhance its enzymatic efficiency during DNA synthesis.

Pathways

POLD1 is involved in the DNA replication and repair pathways. Its interaction with replication protein A (RPA) and PCNA facilitates the smooth completion of Okazaki fragment processing within the DNA replication pathway. Additionally POLD1 plays a role in the base excision repair pathway working alongside other repair proteins such as FEN1 (flap endonuclease 1) and LIG1 (DNA ligase I) to correct DNA lesions. These interactions enforce fidelity and speed during genetic information transfer.

Associated diseases and disorders

Mutations or dysregulation of POLD1 have links to various cancers and genomic instability disorders. Somatic mutations in POLD1 are associated with colorectal cancer where the impaired replication and repair capabilities lead to accumulated mutations. Another disorder related to defective POLD1 function is MDPL syndrome (mandibular hypoplasia deafness progeroid features and lipodystrophy) where the genomic instability contributes to the disease phenotype. Through these conditions POLD1 interacts with tumor suppressors and regulatory proteins involved in DNA damage response highlighting its significance in maintaining genomic integrity and preventing disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com