Rabbit Recombinant Monoclonal PCK2 antibody - conjugated to Alkaline Phosphatase.
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.016% Magnesium chloride, 0.001% Zinc chloride
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
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Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed:28955899). Can play an active role in glyceroneogenesis and gluconeogenesis (PubMed:28955899).
PEPCK2, PCK2, PEPCK-M, mtPCK2
Rabbit Recombinant Monoclonal PCK2 antibody - conjugated to Alkaline Phosphatase.
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.016% Magnesium chloride, 0.001% Zinc chloride
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Phosphoenolpyruvate carboxykinase 2 (PCK2) also known as mitochondrial PEPCK is an enzyme that plays an important role in gluconeogenesis. It catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the mitochondria. PCK2 has a molecular mass of approximately 69 kDa and is expressed mainly in tissues that require high energy production such as the liver and kidney. Despite its vital function in energy metabolism PCK2 is also present in other metabolic tissues.
PCK2 contributes to the regulation of glucose homeostasis. It achieves this by being a part of the gluconeogenic pathway working in concert with cytosolic PCK1. PCK2 does not work alone; it integrates into a larger metabolic framework that includes various enzymes essential for converting different substrates into glucose. This enzyme is notably active during fasting states or periods of high energy demands such as intense exercise ensuring a continuous supply of glucose to maintain cellular functions.
PCK2 plays an important role in the gluconeogenesis and TCA cycle. The gluconeogenesis pathway allows the liver and kidney to produce glucose from non-carbohydrate substrates which is integral during fasting and low-carbohydrate diet. It is functionally linked with other key enzymes like pyruvate carboxylase in converting pyruvate to glucose and also cooperates with malate dehydrogenase in the TCA cycle. This cooperation ensures efficient energy production and conversion balance in the cell.
PCK2 expression is linked to type 2 diabetes and certain cancers. In type 2 diabetes altered PCK2 activity impacts glucose production contributing to hyperglycemia. Studies have shown that its dysregulation may correlate with insulin resistance a characteristic feature of diabetes. In cancer particularly some forms of breast cancer the interplay between PCK2 and oncogenes like KRAS can affect tumor metabolism promoting the adaptation of cancer cells to the energy-demanding environment.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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