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AB194384

Anti-AP1M1 antibody

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(2 Publications)

Mouse Polyclonal AP1M1 antibody. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AP1M1 aa 1-100.

View Alternative Names

CLTNM, AP1M1, AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, Clathrin assembly protein complex 1 mu-1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin

2 Images
Western blot - Anti-AP1M1 antibody (AB194384)
  • WB

Supplier Data

Western blot - Anti-AP1M1 antibody (AB194384)

All lanes:

Western blot - Anti-AP1M1 antibody (ab194384) at 1/500 dilution

All lanes:

U-2 OS cell lysate at 50 µg

Predicted band size: 49 kDa

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Western blot - Anti-AP1M1 antibody (AB194384)
  • WB

Supplier Data

Western blot - Anti-AP1M1 antibody (AB194384)

Predicted MW of immunogen : 34.25 KDa

All lanes:

Western blot - Anti-AP1M1 antibody (ab194384) at 1/1000 dilution

All lanes:

recombinant human AP1M1 (immunogen) at 0.2 µg

Predicted band size: 32 kDa,49 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human AP1M1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BXS5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP1M1 also known as Adaptor-related Protein Complex 1 Mu 1 Subunit is a protein with a molecular mass of around 50 kDa. AP1M1 functions as a component of the adaptor protein complex 1 (AP-1). It plays an essential mechanical role in sorting and trafficking proteins by mediating the selection of cargo molecules for transport vesicles. This protein is expressed in multiple tissues with particularly high levels noted in neuronal cells.
Biological function summary

AP1M1 serves as a critical link in intracellular transport processes. It is part of the AP-1 complex which consists of other subunits like gamma beta and sigma. These complexes work together to ensure proteins move correctly within the cell especially between the trans-Golgi network and endosomes. AP1M1 assists in clathrin-mediated trafficking playing an important role in maintaining cellular functionality by organizing the delivery of membrane proteins and other cellular components.

Pathways

AP1M1 integrates into the clathrin-coated vesicle pathway and the endocytic pathway. It cooperates with proteins such as clathrin and adaptins including other AP complexes to facilitate the formation of transport vesicles. These pathways are pivotal for protein sorting and the regulation of cellular homeostasis contributing to processes such as receptor recycling and lysosomal enzyme targeting.

Mutations or dysfunction in AP1M1 are linked with neurodegenerative diseases like Alzheimer's disease and some cancers. AP1M1's interaction with proteins like tau in Alzheimer’s illustrates its importance in neurological pathways and synaptic health. In cancer improper protein sorting due to AP1M1 malfunction may influence metastatic behavior as it is connected to proteins involved in cell growth and division.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
See full target information AP1M1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 61: PubMed36069226

2022

STAT5b is a marker of poor prognosis, rather than a therapeutic target in glioblastomas.

Applications

Unspecified application

Species

Unspecified reactive species

Nadège Dubois,Sharon Berendsen,Katherine Tan,Laurent Schoysmans,Wim Spliet,Tatjana Seute,Vincent Bours,Pierre A Robe

Frontiers in immunology 10:2151 PubMed31572371

2019

Lovastatin Inhibits HIV-1-Induced MHC-I Downregulation by Targeting Nef-AP-1 Complex Formation: A New Strategy to Boost Immune Eradication of HIV-1 Infected Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bingfeng Liu,Xu Zhang,Wanying Zhang,Liyang Wu,Shuliang Jing,Weiwei Liu,Baijin Xia,Fan Zou,Lijuan Lu,Xiancai Ma,Dalian He,Qifei Hu,Yiwen Zhang,Kai Deng,Weiping Cai,Xiaoping Tang,Tao Peng,Hui Zhang,Linghua Li
View all publications
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