AB230273

Anti-AP1M1 antibody

Name: Anti-AP1M1 antibody (ab230273) | Abcam
Brand: Abcam Limited
SKU: AB230273
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal AP1M1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AP1M1 aa 1-200.

View Alternative Names

CLTNM, AP1M1, AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, Clathrin assembly protein complex 1 mu-1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP1M1 antibody (AB230273)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP1M1 antibody (AB230273)

Paraffin-embedded human placenta tissue stained for AP1M1 using ab230273 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP1M1 antibody (AB230273)

Paraffin-embedded human skin tissue stained for AP1M1 using ab230273 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-AP1M1 antibody (AB230273)

All lanes:

Western blot - Anti-AP1M1 antibody (ab230273) at 1/1000 dilution

Lane 1:

A-375 (Human malignant melanoma cell line) whole cell lysate

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human AP1M1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BXS5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP1M1 also known as Adaptor-related Protein Complex 1 Mu 1 Subunit is a protein with a molecular mass of around 50 kDa. AP1M1 functions as a component of the adaptor protein complex 1 (AP-1). It plays an essential mechanical role in sorting and trafficking proteins by mediating the selection of cargo molecules for transport vesicles. This protein is expressed in multiple tissues with particularly high levels noted in neuronal cells.

Biological function summary

AP1M1 serves as a critical link in intracellular transport processes. It is part of the AP-1 complex which consists of other subunits like gamma beta and sigma. These complexes work together to ensure proteins move correctly within the cell especially between the trans-Golgi network and endosomes. AP1M1 assists in clathrin-mediated trafficking playing an important role in maintaining cellular functionality by organizing the delivery of membrane proteins and other cellular components.

Pathways

AP1M1 integrates into the clathrin-coated vesicle pathway and the endocytic pathway. It cooperates with proteins such as clathrin and adaptins including other AP complexes to facilitate the formation of transport vesicles. These pathways are pivotal for protein sorting and the regulation of cellular homeostasis contributing to processes such as receptor recycling and lysosomal enzyme targeting.

Mutations or dysfunction in AP1M1 are linked with neurodegenerative diseases like Alzheimer's disease and some cancers. AP1M1's interaction with proteins like tau in Alzheimer’s illustrates its importance in neurological pathways and synaptic health. In cancer improper protein sorting due to AP1M1 malfunction may influence metastatic behavior as it is connected to proteins involved in cell growth and division.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

See full target information AP1M1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life sciences 229:13-20 PubMed30953643

2019

HIV-1 Nef-GCC185 interaction regulates assembly of cellular protein complexes at TGN targeting MHC-I downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Sushila Kumari,Manjeet Kumar,Richa Verma,Jimut Kanti Ghosh,Raj Kamal Tripathi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AP1M1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP1M1 antibody (AB230273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP1M1 antibody (AB230273)

Western blot - Anti-AP1M1 antibody (AB230273)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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