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AB217055

Anti-AP1S1 antibody

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(1 Publication)

Rabbit Polyclonal AP1S1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human AP1S1 aa 100 to C-terminus.

View Alternative Names

AP19, CLAPS1, AP1S1, AP-1 complex subunit sigma-1A, Adaptor protein complex AP-1 subunit sigma-1A, Adaptor-related protein complex 1 subunit sigma-1A, Clathrin assembly protein complex 1 sigma-1A small chain, Clathrin coat assembly protein AP19, Golgi adaptor HA1/AP1 adaptin sigma-1A subunit, HA1 19 kDa subunit, Sigma 1a subunit of AP-1 clathrin, Sigma-adaptin 1A, Sigma1A-adaptin

2 Images
Western blot - Anti-AP1S1 antibody (AB217055)
  • WB

Supplier Data

Western blot - Anti-AP1S1 antibody (AB217055)

cells prepared using NETN lysis buffer

All lanes:

Western blot - Anti-AP1S1 antibody (ab217055) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 15 µg

Lane 2:

293T cell lysate at 15 µg

Lane 3:

Jurkat cell lysate at 15 µg

Lane 4:

TCMK1 cell lysate at 15 µg

Lane 5:

NIH 3T3 cell lysate at 15 µg

Predicted band size: 19 kDa

true

Exposure time: 10s

Immunoprecipitation - Anti-AP1S1 antibody (AB217055)
  • IP

Unknown

Immunoprecipitation - Anti-AP1S1 antibody (AB217055)

Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling AP1S1 using ab217055 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated AP1S1, ab217055 was used at 1 μg/ml. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-AP1S1 antibody (ab217055)

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human AP1S1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61966

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab217055 was affinity purified using an epitope specific to AP1S1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP1S1 also known as Adaptor Protein complex 1 Sigma 1 is an integral component of the AP-1 adaptor complex. This complex is involved in the sorting and trafficking of transmembrane proteins. AP1S1 has a molecular mass of approximately 19 kDa and is universally expressed in various tissues including the liver and brain. Its main function involves the interaction with clathrin aiding in the selection of cargo proteins for transport between the trans-Golgi network and endosomes.
Biological function summary

AP1S1 plays a significant role in vesicular trafficking processes. It participates as part of the AP-1 adaptor protein complex which is responsible for mediating sorting signals in coated vesicles. Through this participation AP1S1 influences the efficiency and specificity of intracellular transport. The AP-1 complex also interacts with phosphatidylinositol phosphates further regulating the vesicle formation and protein sorting mechanisms within cells. This protein's involvement is critical for maintaining cellular homeostasis and specific protein function.

Pathways

AP1S1 functions in pathways related to protein sorting and vesicular transport. It plays a role within the clathrin-mediated endocytosis pathway important for cellular receptor regulation and nutrient uptake. AP1S1 connects with other proteins such as clathrin and other adaptor proteins to form a network ensuring efficient cargo selection and transportation. This function ensures a streamlined process for the cellular uptake and regulation of essential molecules.

AP1S1 has links to various conditions through its role in protein trafficking. Mutations in AP1S1 associate with Mednik Syndrome a disorder characterized by neurological issues due to improper protein sorting. Another connection involves its association with hereditary spastic paraplegia where misregulation of protein trafficking results in neurological deterioration. In these contexts AP1S1 interacts with proteins like AP1B1 which similarly impacts vesicular transport and sorting pathways affecting overall cellular functionality.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
See full target information AP1S1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human genetics 139:1247-1259 PubMed32306098

2020

AP1S1 missense mutations cause a congenital enteropathy via an epithelial barrier defect.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina M C Klee,Andreas R Janecke,Hasret A Civan,Štefan Rosipal,Peter Heinz-Erian,Lukas A Huber,Thomas Müller,Georg F Vogel
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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