AB97590

Anti-AP1S2 antibody

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(2 Publications)

Rabbit Polyclonal AP1S2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AP1S2 aa 1-150.

View Alternative Names

DC22, AP1S2, AP-1 complex subunit sigma-2, Adaptor protein complex AP-1 subunit sigma-1B, Adaptor-related protein complex 1 subunit sigma-1B, Clathrin assembly protein complex 1 sigma-1B small chain, Golgi adaptor HA1/AP1 adaptin sigma-1B subunit, Sigma 1B subunit of AP-1 clathrin, Sigma-adaptin 1B, Sigma1B-adaptin

1 Images

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Western blot - Anti-AP1S2 antibody (AB97590)

12% SDS-PAGE

All lanes:

Western blot - Anti-AP1S2 antibody (ab97590) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human AP1S2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P56377

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP1S2 also known as the adaptor-related protein complex 1 sigma 2 subunit plays an important role in vesicle-mediated transport. The protein has a mass of approximately 19 kDa. It is a component of the adaptor protein complex 1 (AP-1) which exists in the Golgi apparatus and endosomal membranes. AP1S2 is expressed in various tissues including the brain and testis where it mediates the sorting of the trans-Golgi network to endosomes.

Biological function summary

This protein is important in the efficient packaging of cargo proteins into vesicles. AP1S2 forms part of the AP-1 complex which interacts with clathrin-coated vesicles. This interaction facilitates the recognition of cargo selection signals ensuring proper protein sorting and transport within cells. The AP-1 complex including AP1S2 also regulates trafficking pathways essential for cellular function.

Pathways

AP1S2 associates with clathrin-dependent transport pathways. It has a significant role in the pathway involving the transport of membranes and select proteins between the trans-Golgi network and endosomes. In this context AP1S2 interacts with other proteins like AP1B1 which aids in the binding of cargo molecules required for transport maintaining the efficient flow of vesicular traffic within cells.

AP1S2 has a connection with X-linked intellectual disability (XLID) reflecting its importance in brain function. Mutations in the AP1S2 gene link to cognitive impairments demonstrating its critical role in neural development. Additionally AP1S2 alterations can be associated with neurodegenerative diseases where its dysfunction affects neural pathways. This protein's interaction with clathrin and other adaptor protein complex members could potentially provide insights into therapeutic targets for treating related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

See full target information AP1S2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature microbiology 9:1293-1311 PubMed38622380

2024

Age-specific nasal epithelial responses to SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Maximillian N J Woodall,Ana-Maria Cujba,Kaylee B Worlock,Katie-Marie Case,Tereza Masonou,Masahiro Yoshida,Krzysztof Polanski,Ni Huang,Rik G H Lindeboom,Lira Mamanova,Liam Bolt,Laura Richardson,Batuhan Cakir,Samuel Ellis,Machaela Palor,Thomas Burgoyne,Andreia Pinto,Dale Moulding,Timothy D McHugh,Aarash Saleh,Eliz Kilich,Puja Mehta,Chris O'Callaghan,Jie Zhou,Wendy Barclay,Paolo De Coppi,Colin R Butler,Mario Cortina-Borja,Heloise Vinette,Sunando Roy,Judith Breuer,Rachel C Chambers,Wendy E Heywood,Kevin Mills,Robert E Hynds,Sarah A Teichmann,Kerstin B Meyer,Marko Z Nikolić,Claire M Smith

Human mutation 42:835-847 PubMed33847015

2021

Different types of disease-causing noncoding variants revealed by genomic and gene expression analyses in families with X-linked intellectual disability.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Field,Raman Kumar,Anna Hackett,Sayaka Kayumi,Cheryl A Shoubridge,Lisa J Ewans,Atma M Ivancevic,Tracy Dudding-Byth,Renée Carroll,Thessa Kroes,Alison E Gardner,Patricia Sullivan,Thuong T Ha,Charles E Schwartz,Mark J Cowley,Marcel E Dinger,Elizabeth E Palmer,Louise Christie,Marie Shaw,Tony Roscioli,Jozef Gecz,Mark A Corbett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AP1S2 antibody

Western blot - Anti-AP1S2 antibody (AB97590)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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