AB284730

Anti-AP2 gamma/TFAP2C antibody [EP2693Y] - BSA and Azide free

RabMAb
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Rabbit Monoclonal AP2 gamma/TFAP2C antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Transcription factor AP-2 gamma, AP2-gamma, Activating enhancer-binding protein 2 gamma, Transcription factor ERF-1, TFAP2C

2 Images

Flow Cytometry (Intracellular) - Anti-AP2 gamma/TFAP2C antibody [EP2693Y] - BSA and Azide free (AB284730)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-AP2 gamma/TFAP2C antibody [EP2693Y] - BSA and Azide free (AB284730)

This data was developed using ab75992, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized MCF-7 cells using ab75992 (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2693Y] - BSA and Azide free (AB284730)

This data was developed using ab75992, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2693Y] (<a href='/en-us/products/primary-antibodies/ap2-gamma-tfap2c-antibody-ep2693y-ab75992'>ab75992</a>) at 1/200000 dilution

All lanes:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2693Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 88% homology with cadherin EGF LAG seven-pass G-type receptor 1. Cross-reactivity with this protein has not been confirmed experimentally

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

ab284730 is the carrier-free version of ab75992

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP2 gamma also known as TFAP2C functions as a transcription factor and belongs to the AP-2 family. AP2 gamma has a molecular weight of approximately 50 kDa. It regulates gene expression by binding to DNA sequence-specific sites influencing diverse cellular processes. This protein commonly expresses in tissues like mammary glands and is important in embryonic development. TFAP2C plays a role in differentiation and development across different cell types.

Biological function summary

AP2 gamma acts as a significant regulator of gene networks that control cell growth and differentiation. This protein contributes to transcriptional activation and repression often functioning within multiprotein complexes. It regulates the expression of genes involved in epithelial cell differentiation and morphogenesis. Importantly TFAP2C helps in the development of ectodermal tissues supporting cellular behaviors necessary for proper tissue formation.

Pathways

TFAP2C is involved in pathways that include developmental processes and differentiation. In particular it takes part in the Wnt signaling pathway influencing gene expression patterns that govern development. AP2 gamma interacts with proteins such as beta-catenin within the Wnt pathway modulating cell fate and proliferation. Another important pathway is the estrogen receptor signaling pathway where TFAP2C partners with proteins like ERα to regulate hormone-responsive genes.

AP2 gamma has links to breast cancer and neural tube defects. In breast cancer TFAP2C modulates genes associated with cancer progression and treatment response interacting with proteins like HER2. Mutations or dysregulation of TFAP2C can also lead to developmental anomalies like neural tube defects possibly due to altered gene expression during embryonic development. Understanding these connections provides insight into therapeutic strategies and developmental biology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

See full target information Transcription factor AP-2 gamma

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AP2 gamma/TFAP2C antibody [EP2693Y] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-AP2 gamma/TFAP2C antibody [EP2693Y] - BSA and Azide free (AB284730)

Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2693Y] - BSA and Azide free (AB284730)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise