Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)

Rabbit Recombinant Monoclonal AP2 gamma/TFAP2C antibody. Suitable for IP, ICC/IF, IHC-P, WB, ChIP-seq, ChIC/CUT&RUN-seq and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (AB324045), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	ICC/IF	IHC-P	WB	ChIP-seq	ChIC/CUT&RUN-seq
Mouse	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Expected	Tested	Tested	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 4 µg	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 5 µg	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information Tfap2c

Function

Sequence-specific DNA-binding transcription factor that interacts with cellular enhancer elements to regulate transcription of selected genes, and which plays a key role in early embryonic development (PubMed:38243114, PubMed:8660922). AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions (PubMed:38243114, PubMed:8660922). TFAP2C plays a key role in early embryonic development by regulating both inner cell mass (ICM) and trophectoderm differentiation (PubMed:38243114). At the 8-cell stage, during morula development, controls expression of cell-polarity genes (PubMed:38243114). Upon trophoblast commitment, binds to late trophectoderm genes in blastocysts together with CDX2, and later to extra-embryonic ectoderm genes together with SOX2 (PubMed:38243114). Binds to both closed and open chromatin with other transcription factors (PubMed:38243114).

Additional Targets

Tfap2c

Alternative names

Tcfap2c, Transcription factor AP-2 gamma, AP2-gamma, AP-2.2, Activating enhancer-binding protein 2 gamma

Rabbit Recombinant Monoclonal AP2 gamma/TFAP2C antibody. Suitable for IP, ICC/IF, IHC-P, WB, ChIP-seq, ChIC/CUT&RUN-seq and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR29851-13
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Western blot - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C Western blot staining using rabbit Anti-AP2 gamma/TFAP2C antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: kidney (PMID: 9765260).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 32587258, PMID: 29695788).
The identity of the bands higher than 75kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045) at 1/1000 dilution
Lane 1: Rat-1 (rat embryonic fibroblast) whole cell lysate at 50 µg
Lane 2: Mouse placenta tissue lysate at 50 µg
Lane 3: Mouse kidney tissue lysate at 50 µg
Lane 4: Rat placenta tissue lysate at 50 µg
Lane 5: Rat kidney tissue lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 49 kDa, 51 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C Western blot staining using rabbit Anti-AP2 gamma/TFAP2C antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: MEF (PMID: 32587258).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 32587258, PMID: 29695788).
In lanes 3-4, the lysates were stored at -80degC prior to Western Blotting. The bands beneath the target band (51 kDa) are likely to be degradation products. In lanes 1-2, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
The identity of the bands higher than 75kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045) at 1/1000 dilution
Lane 1: ES-D3 D3 (mouse blastocyst-derived embryonic stem cell) fresh whole cell lysate at 50 µg
Lane 2: MEF (mouse embryo fibroblast) fresh whole cell lysate at 50 µg
Lane 3: ES-D3 D3 (mouse blastocyst-derived embryonic stem cell) frozen whole cell lysate at 50 µg
Lane 4: MEF (mouse embryo fibroblast) frozen whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 49 kDa, 51 kDa, 36 kDa
Exposure time: 8s
Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-AP2 gamma/TFAP2C antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells labelling AP2 gamma/TFAP2C with ab324045 at 1/500 (1.026 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing nuclear staining in ES-D3 [D3] cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Low expression: MEF (PMID: 32587258)
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C Immunocytochemistry/ Immunofluorescence staining of Rat-1 (rat embryonic fibroblast) using rabbit Anti-AP2 gamma/TFAP2C antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat-1 (rat embryonic fibroblast) cells labelling AP2 gamma/TFAP2C with ab324045 at 1/500 (1.026 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing nuclear staining in Rat-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse placenta using rabbit Anti-AP2 gamma/TFAP2C antibody
Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling AP2 gamma/TFAP2C with ab324045 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse placenta (PMID: 9765260). The section was incubated with ab324045 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat placenta using rabbit Anti-AP2 gamma/TFAP2C antibody
Immunohistochemical analysis of paraffin-embedded Rat placenta tissue labeling AP2 gamma/TFAP2C with ab324045 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat placenta. The section was incubated with ab324045 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse kidney using rabbit Anti-AP2 gamma/TFAP2C antibody
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling AP2 gamma/TFAP2C with ab324045 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse kidney (PMID: 9765260). The section was incubated with ab324045 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat kidney using rabbit Anti-AP2 gamma/TFAP2C antibody
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling AP2 gamma/TFAP2C with ab324045 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat kidney. The section was incubated with ab324045 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunoprecipitation - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
AP2 gamma/TFAP2C was immunoprecipitated from 0.35 mg ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) whole cell lysate with ab324045 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324045 at 1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
All lanes: Immunoprecipitation - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045) at 1/1000 dilution
Lane 1: ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) whole cell lysate at 10 µg
Lane 2: ab324045 at 1/30 IP in ES-D3 [D3] whole cell lysate at 10 µg
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab324045 in ES-D3 [D3] whole cell lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 49 kDa, 51 kDa
Exposure time: 180s
ChIP-sequencing - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
Chromatin was prepared from ES-D3 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5x10⁶ cells and 4 µg of ab324045 [EPR29851-13]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
Chromatin was prepared from ES-D3 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5x10⁶ cells and 4 µg of ab324045 [EPR29851-13]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
Chromatin was prepared from ES-D3 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5x10⁶ cells and 4 µg of ab324045 [EPR29851-13]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIC/CUT&RUN sequencing - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells and 5 µg of ab324045 [EPR29851-13]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
ChIC/CUT&RUN sequencing - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells and 5 µg of ab324045 [EPR29851-13]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
ChIC/CUT&RUN sequencing - Anti-AP2 gamma/TFAP2C antibody [EPR29851-13] (ab324045)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells and 5 µg of ab324045 [EPR29851-13]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.