JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248326

Anti-AP2B1 antibody [EPR7567] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal AP2B1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

ADTB2, CLAPB1, AP2B1, AP-2 complex subunit beta, AP105B, Adaptor protein complex AP-2 subunit beta, Adaptor-related protein complex 2 subunit beta, Beta-2-adaptin, Beta-adaptin, Clathrin assembly protein complex 2 beta large chain, Plasma membrane adaptor HA2/AP2 adaptin beta subunit

2 Images
Western blot - Anti-AP2B1 antibody [EPR7567] - BSA and Azide free (AB248326)
  • WB

Unknown

Western blot - Anti-AP2B1 antibody [EPR7567] - BSA and Azide free (AB248326)

This data was developed using ab129168, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AP2B1 antibody [EPR7567] (<a href='/en-us/products/primary-antibodies/ap2b1-antibody-epr7567-ab129168'>ab129168</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 105 kDa

false

OI-RD Scanning - Anti-AP2B1 antibody [EPR7567] - BSA and Azide free (AB248326)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-AP2B1 antibody [EPR7567] - BSA and Azide free (AB248326)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7567

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB21679

Anti-Clathrin heavy chain antibody

Western blot - Anti-Clathrin heavy chain antibody (AB21679)

  • 5
  • 38
Primary Antibodies

AB170955

Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)]

RabMAb|Recombinant

Western blot - Anti-AP-2 complex subunit alpha-1 antibody [EPR7573(2)(B)] (AB170955)

  • 0
  • 0
Primary Antibodies

AB2807

Anti-alpha Adaptin antibody [AC1-M11]

Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AC1-M11] (AB2807)

  • 5
  • 2
Primary Antibodies

AB108311

Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)]

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (AB108311)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab248326 is the carrier-free version of ab129168.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AP2B1 also known as the beta-1 subunit of the adaptor protein complex 2 (AP-2 complex) has a mass of approximately 105 kDa. It plays an important role in clathrin-mediated endocytosis by forming a bridge between clathrin and cargo molecules. The AP-2 complex is essential for selecting and concentrating cargo proteins that are destined for endocytosis. AP2B1 is highly expressed in most tissues with abundant presence in brain tissue suggesting its importance in neural processes.
Biological function summary

AP2B1 functions as a component of the AP-2 complex which is essential for the formation of clathrin-coated pits on the plasma membrane. It contributes to the regulation of receptor-mediated endocytosis a process important for nutrient uptake and signaling receptor regulation. The AP-2 complex binds to the cytoplasmic tails of transmembrane proteins mediating their internalization into cells for degradation or recycling. This highlights AP2B1's role in maintaining cellular homeostasis and responding to environmental signals.

Pathways

Researchers have shown that AP2B1 is actively involved in the endocytic pathway facilitating the internalization of cell surface receptors into vesicles. It is also linked to the sorting of cargo proteins within the endocytosis pathway in tandem with other proteins like calmodulin. The interaction with dynamin regulates the membrane scission events necessary for vesicle release. Both calmodulin and dynamin are important for the successful execution of these pathways where AP2B1 plays a central role.

Disruptions in AP2B1 function link to neurological disorders such as Alzheimer’s disease potentially due to impaired endocytosis and subsequent cellular signaling dysfunctions. Abnormalities in the AP-2 complex including AP2B1 have also been associated with cancers like melanoma where altered receptor endocytosis can lead to uncontrolled cell signaling and proliferation. Both conditions involve pathways where dynamin works closely with AP2B1 hinting at its wider implications in disease progression and therapeutic target potential.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed : 23676497). The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
See full target information AP2B1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com