Rabbit Polyclonal AP2M1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AP2M1 aa 50-300.
Images
Publications
- Blood 138:1359-13722021An alternate covalent form of platelet αIIbβ3 integrin that resides in focal adhesions and has altered function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAster E Pijning,Mitchell T Blyth,Michelle L Coote,Freda Passam,Joyce Chiu,Philip J HoggPubMed 34375384
- Human molecular genetics 30:880-8922021Ap2s1 mutation causes hypercalcaemia in mice and impairs interaction between calcium-sensing receptor and adaptor protein-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFadil M Hannan,Mark Stevenson,Asha L Bayliss,Victoria J Stokes,Michelle Stewart,Kreepa G Kooblall,Caroline M Gorvin,Gemma Codner,Lydia Teboul,Sara Wells,Rajesh V ThakkerPubMed 33729479
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AP2M1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96CW1
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Chicken | Predicted |
| Cow | Predicted |
| Pig | Predicted |
| Xenopus laevis | Predicted |
| Xenopus tropicalis | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Pig, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Component of the adaptor protein complex 2 (AP-2) (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis (PubMed:16581796). AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules (By similarity). AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway (PubMed:19033387). During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs (By similarity). The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2 (PubMed:11877457). The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (PubMed:11877457). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).
Alternative names
AP-2 complex subunit mu, AP-2 mu chain, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu, Clathrin assembly protein complex 2 mu medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, HA2 50 kDa subunit, Plasma membrane adaptor AP-2 50 kDa protein, CLAPM1, KIAA0109, AP2M1
Recommended products
Rabbit Polyclonal AP2M1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AP2M1 aa 50-300.
Alternative names
AP-2 complex subunit mu, AP-2 mu chain, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu, Clathrin assembly protein complex 2 mu medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, HA2 50 kDa subunit, Plasma membrane adaptor AP-2 50 kDa protein, CLAPM1, KIAA0109, AP2M1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AP2M1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96CW1
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
The protein AP2M1 also known as AP-2 complex subunit mu plays an important role in clathrin-mediated endocytosis. It weighs approximately 50 kDa. AP2M1 is mainly expressed in different cell types including neuronal epithelial and fibroblast cells. This protein is part of the adaptor protein (AP) complex family specifically the AP-2 complex which facilitates the recruitment of clathrin and cargo proteins to form clathrin-coated vesicles at the plasma membrane.
- Biological function summary
The AP2M1 protein is involved in the sorting and trafficking of membrane proteins and lipids. It functions as part of the AP-2 complex a heterotetrameric assembly composed of two large adaptins a medium chain (mu) adaptin like AP2M1 and a small chain adaptin. This complex helps recognize and bind specific sorting signals found on the cytosolic tails of transmembrane cargo proteins allowing their incorporation into forming vesicles during endocytosis.
- Pathways
AP2M1 contributes significantly to the endocytic pathway and the regulation of receptor-mediated signaling. Involvement with the insulin signaling pathway highlights its role in internalizing insulin receptors and modulating insulin sensitivity. AP2M1 interacts with other proteins such as clathrin which influences the assembly of clathrin-coated pits at the cell membrane and also with accessory proteins like dynamin facilitating the fission of vesicles during endocytosis.
- Associated diseases and disorders
Research associates AP2M1 with neurodegenerative diseases such as Alzheimer's disease due to its role in synaptic vesicle recycling and endocytosis affecting neuron function. It also relates to metabolic disorders like diabetes as improper function or regulation of AP2M1 can impact insulin receptor internalization and downstream signaling. Other proteins connected to AP2M1 through these disorders include clathrin and dynamin which are pivotal for maintaining the integrity of cellular transport mechanisms.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-AP2M1 antibody (ab137727)
10% SDS PAGE
All lanes: Western blot - Anti-AP2M1 antibody (ab137727) at 1/500 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 49 kDa
Western blot - Anti-AP2M1 antibody (ab137727)
10% SDS PAGE
All lanes: Western blot - Anti-AP2M1 antibody (ab137727) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 49 kDa
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