AB109397

Anti-AP2M1 (phospho T156) antibody [EPR4700]

RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal AP2M1 phospho T156 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

View Alternative Names

CLAPM1, KIAA0109, AP2M1, AP-2 complex subunit mu, AP-2 mu chain, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu, Clathrin assembly protein complex 2 mu medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, HA2 50 kDa subunit, Plasma membrane adaptor AP-2 50 kDa protein

4 Images

Lab

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397)

1%SDS Hot lysis method is preferred to enhance the signal.

All lanes:

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by RIPA lysis method at 20 µg

Lane 2:

C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by RIPA lysis method at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397)

All lanes:

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method. Membrane then treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397)

All lanes:

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

Lane 1:

Untreated C6 (Rat glial tumor glial cell) whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method. Membrane then treated with alkaline phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397)

All lanes:

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM calyculin A for 30 minutes whole cell lysate at 15 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM calyculin A for 30 minutes whole cell lysate. Membrane then treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

Carrier free

Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4700

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein AP2M1 also known as AP-2 complex subunit mu plays an important role in clathrin-mediated endocytosis. It weighs approximately 50 kDa. AP2M1 is mainly expressed in different cell types including neuronal epithelial and fibroblast cells. This protein is part of the adaptor protein (AP) complex family specifically the AP-2 complex which facilitates the recruitment of clathrin and cargo proteins to form clathrin-coated vesicles at the plasma membrane.

Biological function summary

The AP2M1 protein is involved in the sorting and trafficking of membrane proteins and lipids. It functions as part of the AP-2 complex a heterotetrameric assembly composed of two large adaptins a medium chain (mu) adaptin like AP2M1 and a small chain adaptin. This complex helps recognize and bind specific sorting signals found on the cytosolic tails of transmembrane cargo proteins allowing their incorporation into forming vesicles during endocytosis.

Pathways

AP2M1 contributes significantly to the endocytic pathway and the regulation of receptor-mediated signaling. Involvement with the insulin signaling pathway highlights its role in internalizing insulin receptors and modulating insulin sensitivity. AP2M1 interacts with other proteins such as clathrin which influences the assembly of clathrin-coated pits at the cell membrane and also with accessory proteins like dynamin facilitating the fission of vesicles during endocytosis.

Research associates AP2M1 with neurodegenerative diseases such as Alzheimer's disease due to its role in synaptic vesicle recycling and endocytosis affecting neuron function. It also relates to metabolic disorders like diabetes as improper function or regulation of AP2M1 can impact insulin receptor internalization and downstream signaling. Other proteins connected to AP2M1 through these disorders include clathrin and dynamin which are pivotal for maintaining the integrity of cellular transport mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the adaptor protein complex 2 (AP-2) (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis (PubMed : 16581796). AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules (By similarity). AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway (PubMed : 19033387). During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed : 23676497). The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs (By similarity). The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2 (PubMed : 11877457). The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (PubMed : 11877457). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).

See full target information AP2M1 pT156

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39028260

2024

SEL-5/AAK1 regulates cell migration and cell outgrowth independently of its kinase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Filip Knop,Apolena Zounarová,Vojtěch Šabata,Teije Corneel Middelkoop,Marie Macůrková

Science signaling 14: PubMed34315807

2021

Dysregulation of the AP2M1 phosphorylation cycle by LRRK2 impairs endocytosis and leads to dopaminergic neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Qinfang Liu,Judith Bautista-Gomez,Daniel A Higgins,Jianzhong Yu,Yulan Xiong

Neurobiology of disease 141:104948 PubMed32434048

2020

Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

George R Heaton,Natalie Landeck,Adamantios Mamais,Mike A Nalls,Jonathon Nixon-Abell,Ravindran Kumaran,Alexandra Beilina,Laura Pellegrini,Yan Li,Kirsten Harvey,Mark R Cookson

Viruses 12: PubMed31905947

2019

The Serine/Threonine Kinase AP2-Associated Kinase 1 Plays an Important Role in Rabies Virus Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Wang,Jinliang Wang,Lei Shuai,Xiao Ma,Hailin Zhang,Renqiang Liu,Weiye Chen,Xijun Wang,Jinying Ge,Zhiyuan Wen,Zhigao Bu

eLife 8: PubMed31464684

2019

A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Edward A Partlow,Richard W Baker,Gwendolyn M Beacham,Joshua S Chappie,Andres E Leschziner,Gunther Hollopeter

Developmental cell 50:494-508.e11 PubMed31430451

2019

Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Antoni G Wrobel,Zuzana Kadlecova,Jan Kamenicky,Ji-Chun Yang,Torsten Herrmann,Bernard T Kelly,Airlie J McCoy,Philip R Evans,Stephen Martin,Stefan Müller,Susanne Salomon,Filip Sroubek,David Neuhaus,Stefan Höning,David J Owen

Cell reports 26:79-93.e8 PubMed30605688

2019

WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop.

Applications

Unspecified application

Species

Unspecified reactive species

Megan J Agajanian,Matthew P Walker,Alison D Axtman,Roberta R Ruela-de-Sousa,D Stephen Serafin,Alex D Rabinowitz,David M Graham,Meagan B Ryan,Tigist Tamir,Yuko Nakamichi,Melissa V Gammons,James M Bennett,Rafael M Couñago,David H Drewry,Jonathan M Elkins,Carina Gileadi,Opher Gileadi,Paulo H Godoi,Nirav Kapadia,Susanne Müller,André S Santiago,Fiona J Sorrell,Carrow I Wells,Oleg Fedorov,Timothy M Willson,William J Zuercher,Michael B Major

eLife 7: PubMed29345618

2018

NECAPs are negative regulators of the AP2 clathrin adaptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Gwendolyn M Beacham,Edward A Partlow,Jeffrey J Lange,Gunther Hollopeter

eLife 3: PubMed25303366

2014

The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Gunther Hollopeter,Jeffrey J Lange,Ying Zhang,Thien N Vu,Mingyu Gu,Michael Ailion,Eric J Lambie,Brian D Slaughter,Jay R Unruh,Laurence Florens,Erik M Jorgensen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AP2M1 (phospho T156) antibody [EPR4700]

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397)

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397)

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397)

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise