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Rabbit Recombinant Monoclonal AP2M1 phospho T156 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

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Images

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397), expandable thumbnail
  • Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397), expandable thumbnail
  • Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397), expandable thumbnail
  • Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (AB109397), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Component of the adaptor protein complex 2 (AP-2) (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis (PubMed:16581796). AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface (PubMed:12694563, PubMed:12952941, PubMed:14745134, PubMed:14985334, PubMed:15473838, PubMed:31104773). AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules (By similarity). AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway (PubMed:19033387). During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs (By similarity). The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2 (PubMed:11877457). The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (PubMed:11877457). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).

Alternative names

CLAPM1, KIAA0109, KIAA0109, CLAPM1, AP2M1, AP-2 complex subunit mu, AP-2 mu chain, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu, Clathrin assembly protein complex 2 mu medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, HA2 50 kDa subunit, Plasma membrane adaptor AP-2 50 kDa protein

Recommended products

Rabbit Recombinant Monoclonal AP2M1 phospho T156 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4700

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein AP2M1 also known as AP-2 complex subunit mu plays an important role in clathrin-mediated endocytosis. It weighs approximately 50 kDa. AP2M1 is mainly expressed in different cell types including neuronal epithelial and fibroblast cells. This protein is part of the adaptor protein (AP) complex family specifically the AP-2 complex which facilitates the recruitment of clathrin and cargo proteins to form clathrin-coated vesicles at the plasma membrane.

Biological function summary

The AP2M1 protein is involved in the sorting and trafficking of membrane proteins and lipids. It functions as part of the AP-2 complex a heterotetrameric assembly composed of two large adaptins a medium chain (mu) adaptin like AP2M1 and a small chain adaptin. This complex helps recognize and bind specific sorting signals found on the cytosolic tails of transmembrane cargo proteins allowing their incorporation into forming vesicles during endocytosis.

Pathways

AP2M1 contributes significantly to the endocytic pathway and the regulation of receptor-mediated signaling. Involvement with the insulin signaling pathway highlights its role in internalizing insulin receptors and modulating insulin sensitivity. AP2M1 interacts with other proteins such as clathrin which influences the assembly of clathrin-coated pits at the cell membrane and also with accessory proteins like dynamin facilitating the fission of vesicles during endocytosis.

Associated diseases and disorders

Research associates AP2M1 with neurodegenerative diseases such as Alzheimer's disease due to its role in synaptic vesicle recycling and endocytosis affecting neuron function. It also relates to metabolic disorders like diabetes as improper function or regulation of AP2M1 can impact insulin receptor internalization and downstream signaling. Other proteins connected to AP2M1 through these disorders include clathrin and dynamin which are pivotal for maintaining the integrity of cellular transport mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397), expandable thumbnail

    Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)

    1%SDS Hot lysis method is preferred to enhance the signal.

    All lanes: Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by RIPA lysis method

    Lane 2: C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by RIPA lysis method

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa

    1%SDS Hot lysis method is preferred to enhance the signal.

    This data was developed using ab109397, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397), expandable thumbnail

    Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)

    All lanes: Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

    Lane 1: Untreated C6 (Rat glial tumor glial cell) whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

    Lane 2: C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

    Lane 3: C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method. Membrane then treated with alkaline phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa

    This data was developed using ab109397, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397), expandable thumbnail

    Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)

    All lanes: Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM calyculin A for 30 minutes whole cell lysate at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM calyculin A for 30 minutes whole cell lysate. Membrane then treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa

    This data was developed using ab109397, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397), expandable thumbnail

    Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397)

    All lanes: Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution

    Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method. Membrane then treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa

    This data was developed using ab109397, the same antibody clone in a different buffer formulation.

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