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AB247848

Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal AP2M1 phospho T156 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

CLAPM1, KIAA0109, AP2M1, AP-2 complex subunit mu, AP-2 mu chain, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu, Clathrin assembly protein complex 2 mu medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, HA2 50 kDa subunit, Plasma membrane adaptor AP-2 50 kDa protein

4 Images
Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (AB247848)
  • WB

Lab

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (AB247848)

1%SDS Hot lysis method is preferred to enhance the signal.

This data was developed using ab109397, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (<a href='/en-us/products/primary-antibodies/ap2m1-phospho-t156-antibody-epr4700-ab109397'>ab109397</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by RIPA lysis method at 20 µg

Lane 2:

C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by RIPA lysis method at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (AB247848)
  • WB

Unknown

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (AB247848)

This data was developed using ab109397, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (<a href='/en-us/products/primary-antibodies/ap2m1-phospho-t156-antibody-epr4700-ab109397'>ab109397</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method. Membrane then treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (AB247848)
  • WB

Unknown

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (AB247848)

This data was developed using ab109397, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (<a href='/en-us/products/primary-antibodies/ap2m1-phospho-t156-antibody-epr4700-ab109397'>ab109397</a>) at 1/1000 dilution

Lane 1:

Untreated C6 (Rat glial tumor glial cell) whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method. Membrane then treated with alkaline phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (AB247848)
  • WB

Unknown

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] - BSA and Azide free (AB247848)

This data was developed using ab109397, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AP2M1 (phospho T156) antibody [EPR4700] (<a href='/en-us/products/primary-antibodies/ap2m1-phospho-t156-antibody-epr4700-ab109397'>ab109397</a>) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM calyculin A for 30 minutes whole cell lysate at 15 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM calyculin A for 30 minutes whole cell lysate. Membrane then treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4700

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247848 is the carrier-free version of ab109397.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein AP2M1 also known as AP-2 complex subunit mu plays an important role in clathrin-mediated endocytosis. It weighs approximately 50 kDa. AP2M1 is mainly expressed in different cell types including neuronal epithelial and fibroblast cells. This protein is part of the adaptor protein (AP) complex family specifically the AP-2 complex which facilitates the recruitment of clathrin and cargo proteins to form clathrin-coated vesicles at the plasma membrane.
Biological function summary

The AP2M1 protein is involved in the sorting and trafficking of membrane proteins and lipids. It functions as part of the AP-2 complex a heterotetrameric assembly composed of two large adaptins a medium chain (mu) adaptin like AP2M1 and a small chain adaptin. This complex helps recognize and bind specific sorting signals found on the cytosolic tails of transmembrane cargo proteins allowing their incorporation into forming vesicles during endocytosis.

Pathways

AP2M1 contributes significantly to the endocytic pathway and the regulation of receptor-mediated signaling. Involvement with the insulin signaling pathway highlights its role in internalizing insulin receptors and modulating insulin sensitivity. AP2M1 interacts with other proteins such as clathrin which influences the assembly of clathrin-coated pits at the cell membrane and also with accessory proteins like dynamin facilitating the fission of vesicles during endocytosis.

Research associates AP2M1 with neurodegenerative diseases such as Alzheimer's disease due to its role in synaptic vesicle recycling and endocytosis affecting neuron function. It also relates to metabolic disorders like diabetes as improper function or regulation of AP2M1 can impact insulin receptor internalization and downstream signaling. Other proteins connected to AP2M1 through these disorders include clathrin and dynamin which are pivotal for maintaining the integrity of cellular transport mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the adaptor protein complex 2 (AP-2) (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis (PubMed : 16581796). AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface (PubMed : 12694563, PubMed : 12952941, PubMed : 14745134, PubMed : 14985334, PubMed : 15473838, PubMed : 31104773). AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules (By similarity). AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway (PubMed : 19033387). During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed : 23676497). The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs (By similarity). The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2 (PubMed : 11877457). The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (PubMed : 11877457). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).
See full target information AP2M1 phospho T156
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang
View all publications
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