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Rabbit Recombinant Monoclonal AP2S1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950), expandable thumbnail
  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950), expandable thumbnail
  • Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (AB128950), expandable thumbnail
  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950), expandable thumbnail
  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (AB128950), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Not recommended
Expected
Rat
Expected
Not recommended
Tested
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). May also play a role in extracellular calcium homeostasis.

Alternative names

AP17, CLAPS2, AP2S1, AP-2 complex subunit sigma, Adaptor protein complex AP-2 subunit sigma, Adaptor-related protein complex 2 subunit sigma, Clathrin assembly protein 2 sigma small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, HA2 17 kDa subunit, Plasma membrane adaptor AP-2 17 kDa protein, Sigma2-adaptin

Recommended products

Rabbit Recombinant Monoclonal AP2S1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2697(2)
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

AP2S1 also known as adaptor-related protein complex 2 sigma 1 subunit is a protein with an estimated molecular mass of approximately 20 kDa. It is expressed in various human tissues but shows higher expression in the brain. This protein is a component of the adaptor protein 2 (AP-2) complex which plays an important role in clathrin-mediated endocytosis a process essential for the internalization of various molecules into the cell.

Biological function summary

The AP2S1 protein functions as part of the AP-2 complex which regulates the recruitment of clathrin to the plasma membrane facilitating the formation of clathrin-coated vesicles. These vesicles help in the transport of transferrin and its receptor and also influence the internalization of membrane proteins and lipids. This efficient transport system supports cellular activities like receptor recycling and nutrient uptake making it an important participant in cellular homeostasis.

Pathways

AP2S1 is involved in endocytic and vesicular transport pathways. It plays a role in the endocytic trafficking pathway that includes the involvement of other proteins like clathrin and AP2B1 which work together to manage cellular intake of nutrients and receptor-ligand complexes. The AP-2 complex functions as a bridge between cargo proteins and the clathrin lattice during the formation of transport vesicles controlling vital cellular processes.

Associated diseases and disorders

AP2S1 has implications in conditions like familial hypocalciuric hypercalcemia type 3 (FHH3) and Brooks-Widemann Syndrome. Mutations in the AP2S1 gene disrupt the normal calcium-sensing receptor pathway which leads to altered calcium homeostasis manifesting as hypercalcemia in patients with FHH3. This condition links AP2S1 functionally with proteins involved in calcium regulation highlighting its significance in maintaining cellular and systemic equilibrium.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1:100 dilution (12.07 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    14% gel is preferred to 4-20% gradient gel

    All lanes: Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution

    Lane 1: Human fetal brain lysates at 15 µg

    Lane 2: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 4: Mouse brain lysates at 15 µg

    Lane 5: Mouse kidney lysates at 15 µg

    Lane 6: Rat brain lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    ab128950 (purified) at 1:60 dilution (2μg) immunoprecipitating AP2S1 in Human fetal brain lysate.
    Lane 1 (input): Human fetal brain lysate 10μg
    Lane 2 (+): ab128950 & Human fetal brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab128950 in Human fetal brain lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    Predicted band size: 17 kDa

  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    All lanes: Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution

    All lanes: Mouse kidney lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    All lanes: Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/5000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: Mouse brain lysates at 15 µg

    Lane 3: Rat brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    All lanes: Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution

    Lane 1: Fetal brain lysate at 10 µg

    Lane 2: SH-SY5Y lysate at 10 µg

    Lane 3: 293T lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1:100 dilution (12.07 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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Product protocols

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